2vj3: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
== | |||
==Human Notch-1 EGFs 11-13== | |||
<StructureSection load='2vj3' size='340' side='right' caption='[[2vj3]], [[Resolution|resolution]] 2.60Å' scene=''> | <StructureSection load='2vj3' size='340' side='right' caption='[[2vj3]], [[Resolution|resolution]] 2.60Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| Line 5: | Line 6: | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1toz|1toz]], [[2f8y|2f8y]], [[1yyh|1yyh]], [[1pb5|1pb5]], [[2f8x|2f8x]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1toz|1toz]], [[2f8y|2f8y]], [[1yyh|1yyh]], [[1pb5|1pb5]], [[2f8x|2f8x]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vj3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vj3 OCA], [http://pdbe.org/2vj3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vj3 RCSB], [http://www.ebi.ac.uk/pdbsum/2vj3 PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vj3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vj3 OCA], [http://pdbe.org/2vj3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vj3 RCSB], [http://www.ebi.ac.uk/pdbsum/2vj3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2vj3 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
| Line 38: | Line 39: | ||
[[Category: Activator]] | [[Category: Activator]] | ||
[[Category: Ank repeat]] | [[Category: Ank repeat]] | ||
[[Category: Calcium]] | |||
[[Category: Developmental protein]] | [[Category: Developmental protein]] | ||
[[Category: Differentiation]] | [[Category: Differentiation]] | ||
| Line 51: | Line 53: | ||
[[Category: Nucleus]] | [[Category: Nucleus]] | ||
[[Category: Phosphorylation]] | [[Category: Phosphorylation]] | ||
[[Category: Polymorphism]] | |||
[[Category: Receptor]] | [[Category: Receptor]] | ||
[[Category: Signalling]] | [[Category: Signalling]] | ||
Revision as of 10:25, 16 November 2017
Human Notch-1 EGFs 11-13Human Notch-1 EGFs 11-13
Structural highlights
Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedThe Notch receptor and its ligands are key components in a core metazoan signaling pathway that regulates the spatial patterning, timing and outcome of many cell-fate decisions. Ligands contain a disulfide-rich Delta/Serrate/LAG-2 (DSL) domain required for Notch trans-activation or cis-inhibition. Here we report the X-ray structure of a receptor binding region of a Notch ligand, the DSL-EGF3 domains of human Jagged-1 (J-1(DSL-EGF3)). The structure reveals a highly conserved face of the DSL domain, and we show, by functional analysis of Drosophila melanogster ligand mutants, that this surface is required for both cis- and trans-regulatory interactions with Notch. We also identify, using NMR, a surface of Notch-1 involved in J-1(DSL-EGF3) binding. Our data imply that cis- and trans-regulation may occur through the formation of structurally distinct complexes that, unexpectedly, involve the same surfaces on both ligand and receptor. A conserved face of the Jagged/Serrate DSL domain is involved in Notch trans-activation and cis-inhibition.,Cordle J, Johnson S, Tay JZ, Roversi P, Wilkin MB, de Madrid BH, Shimizu H, Jensen S, Whiteman P, Jin B, Redfield C, Baron M, Lea SM, Handford PA Nat Struct Mol Biol. 2008 Aug;15(8):849-57. Epub 2008 Jul 27. PMID:18660822[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
|
| ||||||||||||||||||
Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)
OCA- Human
- Cordle, J
- Johnson, S
- Lea, S M
- Roversi, P
- Tay, J Z
- Activator
- Ank repeat
- Calcium
- Developmental protein
- Differentiation
- Egf
- Egf-like domain
- Extracellular
- Glycoprotein
- Jagged
- Membrane
- Metal-binding
- Notch
- Notch signaling pathway
- Nucleus
- Phosphorylation
- Polymorphism
- Receptor
- Signalling
- Transcription
- Transcription regulation
- Transmembrane