1q97: Difference between revisions
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==The structure of the Saccharomyces cerevisiae SR protein kinase, Sky1p, with bound ATP== | ==The structure of the Saccharomyces cerevisiae SR protein kinase, Sky1p, with bound ATP== | ||
<StructureSection load='1q97' size='340' side='right' caption='[[1q97]], [[Resolution|resolution]] 2.30Å' scene=''> | <StructureSection load='1q97' size='340' side='right' caption='[[1q97]], [[Resolution|resolution]] 2.30Å' scene=''> | ||
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1q8y|1q8y]], [[1q8z|1q8z]], [[1q99|1q99]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1q8y|1q8y]], [[1q8z|1q8z]], [[1q99|1q99]]</td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SKY1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr> | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SKY1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q97 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q97 OCA], [http://pdbe.org/1q97 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1q97 RCSB], [http://www.ebi.ac.uk/pdbsum/1q97 PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q97 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q97 OCA], [http://pdbe.org/1q97 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1q97 RCSB], [http://www.ebi.ac.uk/pdbsum/1q97 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1q97 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
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<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
</jmolCheckbox> | </jmolCheckbox> | ||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/ | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1q97 ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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</div> | </div> | ||
<div class="pdbe-citations 1q97" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 1q97" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 05:05, 16 November 2017
The structure of the Saccharomyces cerevisiae SR protein kinase, Sky1p, with bound ATPThe structure of the Saccharomyces cerevisiae SR protein kinase, Sky1p, with bound ATP
Structural highlights
Function[SKY1_YEAST] Constitutively active kinase, specifically and sequentially phosphorylates serine/arginine (SR)-type shuttling mRNA binding proteins in their RS dipeptide repeats.[1] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedConformational changes are thought to play a key role in the function of active protein kinases, although little is known about how these changes relate to the mechanism of phosphorylation. Here we present four high-resolution structures of a single crystal form of Sky1p, a constitutively active serine kinase implicated in yeast RNA processing, each in a different state of nucleotide binding. By comparing the apoenzyme structure to the ADP- and ATP-bound Sky1p structures, we have revealed conformational changes caused by ATP binding or conversion from nucleotide reactant to product. Rotation of the small lobe of the kinase closes the cleft upon binding, allowing the nucleotide to interact with residues from both lobes of the kinase, although some interactions thought to be important for phosphotransfer are missing in the ATP-containing structure. In the apoenzyme, a kinase-conserved phosphate-anchoring loop is in a twisted conformation that is incompatible with ADP and ATP binding, providing a potential mechanism for facilitating ADP release in Sky1p. The nonhydrolyzable ATP analogue AMP-PNP binds in a unique mode that fails to induce lobe closure. This observation, along with comparisons between the two independent molecules in the asymmetric unit of each structure, has provided new molecular details about how the nucleotide binds and induces closure. Finally, we have used mutational analysis to establish the importance of a glycine within the linker that connects the two lobes of Sky1p. Nucleotide-induced conformational changes in the Saccharomyces cerevisiae SR protein kinase, Sky1p, revealed by X-ray crystallography.,Nolen B, Ngo J, Chakrabarti S, Vu D, Adams JA, Ghosh G Biochemistry. 2003 Aug 19;42(32):9575-85. PMID:12911299[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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