1a77: Difference between revisions
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==FLAP ENDONUCLEASE-1 FROM METHANOCOCCUS JANNASCHII== | ==FLAP ENDONUCLEASE-1 FROM METHANOCOCCUS JANNASCHII== | ||
<StructureSection load='1a77' size='340' side='right' caption='[[1a77]], [[Resolution|resolution]] 2.00Å' scene=''> | <StructureSection load='1a77' size='340' side='right' caption='[[1a77]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
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<table><tr><td colspan='2'>[[1a77]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A77 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A77 FirstGlance]. <br> | <table><tr><td colspan='2'>[[1a77]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A77 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A77 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a77 OCA], [http://pdbe.org/1a77 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1a77 RCSB], [http://www.ebi.ac.uk/pdbsum/1a77 PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a77 OCA], [http://pdbe.org/1a77 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1a77 RCSB], [http://www.ebi.ac.uk/pdbsum/1a77 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1a77 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
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</div> | </div> | ||
<div class="pdbe-citations 1a77" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 1a77" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 13:48, 15 November 2017
FLAP ENDONUCLEASE-1 FROM METHANOCOCCUS JANNASCHIIFLAP ENDONUCLEASE-1 FROM METHANOCOCCUS JANNASCHII
Structural highlights
Function[FEN_METJA] Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double-stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedFlap endonuclease-1 (FEN-1), a structure specific nuclease, is an essential enzyme for eukaryotic DNA replication and repair. The crystal structure of FEN-1 from Methanococcus jannaschii, determined at 2.0 A resolution, reveals an active site with two metal ions residing on top of a deep cleft where several conserved acidic residues are clustered. Near the active site, a long flexible loop comprised of many basic and aromatic residues forms a hole large enough to accommodate the DNA substrate. Deletion mutations in this loop significantly decreased the nuclease activity and specificity of FEN-1, suggesting that the loop is critical for recognition and cleavage of the junction between single and double-stranded regions of flap DNA. The crystal structure of flap endonuclease-1 from Methanococcus jannaschii.,Hwang KY, Baek K, Kim HY, Cho Y Nat Struct Biol. 1998 Aug;5(8):707-13. PMID:9699635[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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