4mb8: Difference between revisions
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<StructureSection load='4mb8' size='340' side='right' caption='[[4mb8]], [[Resolution|resolution]] 2.40Å' scene=''> | <StructureSection load='4mb8' size='340' side='right' caption='[[4mb8]], [[Resolution|resolution]] 2.40Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4mb8]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[4mb8]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Unclassified_mammalia Unclassified mammalia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MB8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MB8 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Phylogenetically predicted sequence for the mammalian uricase ancestor, UOX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Phylogenetically predicted sequence for the mammalian uricase ancestor, UOX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1002697 unclassified Mammalia])</td></tr> | ||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Factor_independent_urate_hydroxylase Factor independent urate hydroxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.3.3 1.7.3.3] </span></td></tr> | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Factor_independent_urate_hydroxylase Factor independent urate hydroxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.3.3 1.7.3.3] </span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mb8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mb8 OCA], [http://pdbe.org/4mb8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4mb8 RCSB], [http://www.ebi.ac.uk/pdbsum/4mb8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4mb8 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mb8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mb8 OCA], [http://pdbe.org/4mb8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4mb8 RCSB], [http://www.ebi.ac.uk/pdbsum/4mb8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4mb8 ProSAT]</span></td></tr> | ||
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</div> | </div> | ||
<div class="pdbe-citations 4mb8" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 4mb8" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Factor independent urate hydroxylase]] | [[Category: Factor independent urate hydroxylase]] | ||
[[Category: Unclassified mammalia]] | |||
[[Category: Murphy, M N]] | [[Category: Murphy, M N]] | ||
[[Category: Ortlund, E O]] | [[Category: Ortlund, E O]] | ||
Revision as of 12:48, 15 November 2017
Evolutionary history and metabolic insights of ancient mammalian uricasesEvolutionary history and metabolic insights of ancient mammalian uricases
Structural highlights
Function[URIC_CANFA] Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin. Publication Abstract from PubMedUricase is an enzyme involved in purine catabolism and is found in all three domains of life. Curiously, uricase is not functional in some organisms despite its role in converting highly insoluble uric acid into 5-hydroxyisourate. Of particular interest is the observation that apes, including humans, cannot oxidize uric acid, and it appears that multiple, independent evolutionary events led to the silencing or pseudogenization of the uricase gene in ancestral apes. Various arguments have been made to suggest why natural selection would allow the accumulation of uric acid despite the physiological consequences of crystallized monosodium urate acutely causing liver/kidney damage or chronically causing gout. We have applied evolutionary models to understand the history of primate uricases by resurrecting ancestral mammalian intermediates before the pseudogenization events of this gene family. Resurrected proteins reveal that ancestral uricases have steadily decreased in activity since the last common ancestor of mammals gave rise to descendent primate lineages. We were also able to determine the 3D distribution of amino acid replacements as they accumulated during evolutionary history by crystallizing a mammalian uricase protein. Further, ancient and modern uricases were stably transfected into HepG2 liver cells to test one hypothesis that uricase pseudogenization allowed ancient frugivorous apes to rapidly convert fructose into fat. Finally, pharmacokinetics of an ancient uricase injected in rodents suggest that our integrated approach provides the foundation for an evolutionarily-engineered enzyme capable of treating gout and preventing tumor lysis syndrome in human patients. Evolutionary history and metabolic insights of ancient mammalian uricases.,Kratzer JT, Lanaspa MA, Murphy MN, Cicerchi C, Graves CL, Tipton PA, Ortlund EA, Johnson RJ, Gaucher EA Proc Natl Acad Sci U S A. 2014 Mar 11;111(10):3763-8. doi:, 10.1073/pnas.1320393111. Epub 2014 Feb 18. PMID:24550457[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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