2age: Difference between revisions
No edit summary |
No edit summary |
||
| Line 4: | Line 4: | ||
|PDB= 2age |SIZE=350|CAPTION= <scene name='initialview01'>2age</scene>, resolution 1.150Å | |PDB= 2age |SIZE=350|CAPTION= <scene name='initialview01'>2age</scene>, resolution 1.150Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SIN:SUCCINIC+ACID'>SIN</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span> | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY=[[2agg|2AGG]], [[2agi|2AGI]], [[2ah4|2AH4]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2age FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2age OCA], [http://www.ebi.ac.uk/pdbsum/2age PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2age RCSB]</span> | |||
}} | }} | ||
| Line 27: | Line 30: | ||
[[Category: Lu, C J.]] | [[Category: Lu, C J.]] | ||
[[Category: Radisky, E S.]] | [[Category: Radisky, E S.]] | ||
[[Category: | [[Category: acyl-enzyme]] | ||
[[Category: | [[Category: hydrolase]] | ||
[[Category: peptidase]] | |||
[[Category: proteinase]] | |||
[[Category: serine protease]] | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:52:17 2008'' | ||
Revision as of 01:52, 31 March 2008
| |||||||
| , resolution 1.150Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Activity: | Trypsin, with EC number 3.4.21.4 | ||||||
| Related: | 2AGG, 2AGI, 2AH4
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Succinyl-AAPR-trypsin acyl-enzyme at 1.15 A resolution
OverviewOverview
Atomic resolution structures of trypsin acyl-enzymes and a tetrahedral intermediate analog, along with previously solved structures representing the Michaelis complex, are used to reconstruct events in the catalytic cycle of this classic serine protease. Structural comparisons provide insight into active site adjustments involved in catalysis. Subtle motions of the catalytic serine and histidine residues coordinated with translation of the substrate reaction center are seen to favor the forward progress of the acylation reaction. The structures also clarify the attack trajectory of the hydrolytic water in the deacylation reaction.
About this StructureAbout this Structure
2AGE is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
ReferenceReference
Insights into the serine protease mechanism from atomic resolution structures of trypsin reaction intermediates., Radisky ES, Lee JM, Lu CJ, Koshland DE Jr, Proc Natl Acad Sci U S A. 2006 May 2;103(18):6835-40. Epub 2006 Apr 24. PMID:16636277
Page seeded by OCA on Mon Mar 31 01:52:17 2008