1a5q: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==P93A VARIANT OF BOVINE PANCREATIC RIBONUCLEASE A== | ==P93A VARIANT OF BOVINE PANCREATIC RIBONUCLEASE A== | ||
<StructureSection load='1a5q' size='340' side='right' caption='[[1a5q]], [[Resolution|resolution]] 2.30Å' scene=''> | <StructureSection load='1a5q' size='340' side='right' caption='[[1a5q]], [[Resolution|resolution]] 2.30Å' scene=''> | ||
| Line 5: | Line 6: | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] </span></td></tr> | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] </span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a5q OCA], [http://pdbe.org/1a5q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1a5q RCSB], [http://www.ebi.ac.uk/pdbsum/1a5q PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a5q OCA], [http://pdbe.org/1a5q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1a5q RCSB], [http://www.ebi.ac.uk/pdbsum/1a5q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1a5q ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| Line 30: | Line 31: | ||
==See Also== | ==See Also== | ||
*[[Temp|Temp]] | *[[Temp|Temp]] | ||
== References == | == References == | ||
Revision as of 13:44, 8 November 2017
P93A VARIANT OF BOVINE PANCREATIC RIBONUCLEASE AP93A VARIANT OF BOVINE PANCREATIC RIBONUCLEASE A
Structural highlights
Function[RNAS1_BOVIN] Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA.[1] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedThe Tyr92-Pro93 peptide group of bovine pancreatic ribonuclease A (RNase A) exists in the cis conformation in the native state. From unfolding/refolding kinetic studies of the disulfide-intact wild-type protein and of a variant in which Pro93 had been replaced by Ala, it had been suggested that the Tyr92-Ala93 peptide group also exists in the cis conformation in the native state. Here, we report the crystal structure of the P93A variant. Although there is disorder in the region of residues 92 and 93, the best structural model contains a cis peptide at this position, lending support to the results of the kinetics experiments. We also report the crystal structure of the C[40, 95]A variant, which is an analog of the major rate-determining three-disulfide intermediate in the oxidative folding of RNase A, missing the 40-95 disulfide bond. As had been detected by NMR spectroscopy, the crystal structure of this analog shows disorder in the region surrounding the missing disulfide. However, the global chain fold of the remainder of the protein, including the disulfide bond between Cys65 and Cys72, appears to be unaffected by the mutation. Crystal structures of two mutants that have implications for the folding of bovine pancreatic ribonuclease A.,Pearson MA, Karplus PA, Dodge RW, Laity JH, Scheraga HA Protein Sci. 1998 May;7(5):1255-8. PMID:9605332[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
|
| ||||||||||||||||||