2a97: Difference between revisions
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|PDB= 2a97 |SIZE=350|CAPTION= <scene name='initialview01'>2a97</scene>, resolution 1.80Å | |PDB= 2a97 |SIZE=350|CAPTION= <scene name='initialview01'>2a97</scene>, resolution 1.80Å | ||
|SITE= <scene name='pdbsite=CDA:First+Cd+Binding+Site'>CDA</scene>, <scene name='pdbsite=CDB:Second+Cd+Binding+Site'>CDB</scene>, <scene name='pdbsite=CDC:Third+Cd+Binding+Site'>CDC</scene>, <scene name='pdbsite=CDD:Fourt+Cd+Binding+Site'>CDD</scene>, <scene name='pdbsite=CDE:Fifth+Cd+Binding+Site'>CDE</scene>, <scene name='pdbsite=CDF:Sixth+Cd+Binding+Site'>CDF</scene>, <scene name='pdbsite=ZNA:First+Zn+Binding+Site'>ZNA</scene> and <scene name='pdbsite=ZNB:Second+Zn+Binding+Site'>ZNB</scene> | |SITE= <scene name='pdbsite=CDA:First+Cd+Binding+Site'>CDA</scene>, <scene name='pdbsite=CDB:Second+Cd+Binding+Site'>CDB</scene>, <scene name='pdbsite=CDC:Third+Cd+Binding+Site'>CDC</scene>, <scene name='pdbsite=CDD:Fourt+Cd+Binding+Site'>CDD</scene>, <scene name='pdbsite=CDE:Fifth+Cd+Binding+Site'>CDE</scene>, <scene name='pdbsite=CDF:Sixth+Cd+Binding+Site'>CDF</scene>, <scene name='pdbsite=ZNA:First+Zn+Binding+Site'>ZNA</scene> and <scene name='pdbsite=ZNB:Second+Zn+Binding+Site'>ZNB</scene> | ||
|LIGAND= <scene name='pdbligand= | |LIGAND= <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span> | ||
|GENE= botF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 Clostridium botulinum]) | |GENE= botF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 Clostridium botulinum]) | ||
|DOMAIN= | |||
|RELATEDENTRY=[[2a8a|2A8A]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a97 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a97 OCA], [http://www.ebi.ac.uk/pdbsum/2a97 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2a97 RCSB]</span> | |||
}} | }} | ||
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[[Category: Binz, T.]] | [[Category: Binz, T.]] | ||
[[Category: Swaminathan, S.]] | [[Category: Swaminathan, S.]] | ||
[[Category: | [[Category: catalytic domain]] | ||
[[Category: | [[Category: clostridium botulinum neurotoxin serotype f]] | ||
[[Category: | [[Category: crystal structure]] | ||
[[Category: light chain]] | |||
[[Category: x-ray]] | |||
[[Category: zinc endopeptidase]] | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:49:35 2008'' | ||
Revision as of 01:49, 31 March 2008
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| , resolution 1.80Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | , , , , , , and | ||||||
| Ligands: | , | ||||||
| Gene: | botF (Clostridium botulinum) | ||||||
| Activity: | Bontoxilysin, with EC number 3.4.24.69 | ||||||
| Related: | 2A8A
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of catalytic domain of Clostridium botulinum neurotoxin serotype F
OverviewOverview
The seven serologically distinct Clostridium botulinum neurotoxins (BoNTs A-G) are zinc endopeptidases which block the neurotransmitter release by cleaving one of the three proteins of the soluble N-ethylmaleimide-sensitive-factor attachment protein receptor complex (SNARE complex) essential for the fusion of vesicles containing neurotransmitters with target membranes. These metallopeptidases exhibit unique specificity for the substrates and peptide bonds they cleave. Development of countermeasures and therapeutics for BoNTs is a priority because of their extreme toxicity and potential misuse as biowarfare agents. Though they share sequence homology and structural similarity, the structural information on each one of them is required to understand the mechanism of action of all of them because of their specificity. Unraveling the mechanism will help in the ultimate goal of developing inhibitors as antibotulinum drugs for the toxins. Here, we report the high-resolution structure of active BoNT/F catalytic domain in two crystal forms. The structure was exploited for modeling the substrate binding and identifying the S1' subsite and the putative exosites which are different from BoNT/A or BoNT/B. The orientation of docking of the substrate at the active site is consistent with the experimental BoNT/A-LC:SNAP-25 peptide model and our proposed model for BoNT/E-LC:SNAP-25.
About this StructureAbout this Structure
2A97 is a Single protein structure of sequence from Clostridium botulinum. Full crystallographic information is available from OCA.
ReferenceReference
Structural analysis of botulinum neurotoxin serotype F light chain: implications on substrate binding and inhibitor design., Agarwal R, Binz T, Swaminathan S, Biochemistry. 2005 Sep 6;44(35):11758-65. PMID:16128577
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