2a9j: Difference between revisions
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|PDB= 2a9j |SIZE=350|CAPTION= <scene name='initialview01'>2a9j</scene>, resolution 2.00Å | |PDB= 2a9j |SIZE=350|CAPTION= <scene name='initialview01'>2a9j</scene>, resolution 2.00Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=3PG:3-PHOSPHOGLYCERIC ACID'>3PG</scene> | |LIGAND= <scene name='pdbligand=3PG:3-PHOSPHOGLYCERIC+ACID'>3PG</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/Bisphosphoglycerate_mutase Bisphosphoglycerate mutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.2.4 5.4.2.4] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Bisphosphoglycerate_mutase Bisphosphoglycerate mutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.2.4 5.4.2.4] </span> | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY=[[1zst|1ZST]], [[1zto|1ZTO]], [[1zsu|1ZSU]], [[2a17|2A17]], [[2a1p|2A1P]], [[2a1q|2A1Q]], [[1t8p|1T8P]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a9j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a9j OCA], [http://www.ebi.ac.uk/pdbsum/2a9j PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2a9j RCSB]</span> | |||
}} | }} | ||
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==Overview== | ==Overview== | ||
Bisphosphoglycerate mutase is an erythrocyte-specific enzyme catalyzing a series of intermolecular phosphoryl group transfer reactions. Its main function is to synthesize 2,3-bisphosphoglycerate, the allosteric effector of hemoglobin. In this paper, we directly observed real-time motion of the enzyme active site and the substrate during phosphoryl transfer. A series of high resolution crystal structures of human bisphosphoglycerate mutase co-crystallized with 2,3-bisphosphoglycerate, representing different time points in the phosphoryl transfer reaction, were solved. These structures not only clarify the argument concerning the substrate binding mode for this enzyme family but also depict the entire process of the key histidine phosphorylation as a "slow movie". It was observed that the enzyme conformation continuously changed during the different states of the reaction. These results provide direct evidence for an "in line" phosphoryl transfer mechanism, and the roles of some key residues in the phosphoryl transfer process are identified. | Bisphosphoglycerate mutase is an erythrocyte-specific enzyme catalyzing a series of intermolecular phosphoryl group transfer reactions. Its main function is to synthesize 2,3-bisphosphoglycerate, the allosteric effector of hemoglobin. In this paper, we directly observed real-time motion of the enzyme active site and the substrate during phosphoryl transfer. A series of high resolution crystal structures of human bisphosphoglycerate mutase co-crystallized with 2,3-bisphosphoglycerate, representing different time points in the phosphoryl transfer reaction, were solved. These structures not only clarify the argument concerning the substrate binding mode for this enzyme family but also depict the entire process of the key histidine phosphorylation as a "slow movie". It was observed that the enzyme conformation continuously changed during the different states of the reaction. These results provide direct evidence for an "in line" phosphoryl transfer mechanism, and the roles of some key residues in the phosphoryl transfer process are identified. | ||
==About this Structure== | ==About this Structure== | ||
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[[Category: Gong, W.]] | [[Category: Gong, W.]] | ||
[[Category: Wang, Y.]] | [[Category: Wang, Y.]] | ||
[[Category: 3-phosphoglycerate]] | [[Category: 3-phosphoglycerate]] | ||
[[Category: bisphosphoglycerate mutase]] | [[Category: bisphosphoglycerate mutase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:49:42 2008'' | ||
Revision as of 01:49, 31 March 2008
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| , resolution 2.00Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Activity: | Bisphosphoglycerate mutase, with EC number 5.4.2.4 | ||||||
| Related: | 1ZST, 1ZTO, 1ZSU, 2A17, 2A1P, 2A1Q, 1T8P
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Human bisphosphoglycerate mutase complexed with 3-phosphoglycerate (17 days)
OverviewOverview
Bisphosphoglycerate mutase is an erythrocyte-specific enzyme catalyzing a series of intermolecular phosphoryl group transfer reactions. Its main function is to synthesize 2,3-bisphosphoglycerate, the allosteric effector of hemoglobin. In this paper, we directly observed real-time motion of the enzyme active site and the substrate during phosphoryl transfer. A series of high resolution crystal structures of human bisphosphoglycerate mutase co-crystallized with 2,3-bisphosphoglycerate, representing different time points in the phosphoryl transfer reaction, were solved. These structures not only clarify the argument concerning the substrate binding mode for this enzyme family but also depict the entire process of the key histidine phosphorylation as a "slow movie". It was observed that the enzyme conformation continuously changed during the different states of the reaction. These results provide direct evidence for an "in line" phosphoryl transfer mechanism, and the roles of some key residues in the phosphoryl transfer process are identified.
About this StructureAbout this Structure
2A9J is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Seeing the process of histidine phosphorylation in human bisphosphoglycerate mutase., Wang Y, Liu L, Wei Z, Cheng Z, Lin Y, Gong W, J Biol Chem. 2006 Dec 22;281(51):39642-8. Epub 2006 Oct 18. PMID:17052986
Page seeded by OCA on Mon Mar 31 01:49:42 2008