5nlc: Difference between revisions
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<StructureSection load='5nlc' size='340' side='right' caption='[[5nlc]], [[Resolution|resolution]] 1.90Å' scene=''> | <StructureSection load='5nlc' size='340' side='right' caption='[[5nlc]], [[Resolution|resolution]] 1.90Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5nlc]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NLC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NLC FirstGlance]. <br> | <table><tr><td colspan='2'>[[5nlc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Anacystis_nidulans_r2 Anacystis nidulans r2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NLC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NLC FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Synpcc7942_2060 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1140 Anacystis nidulans R2])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nlc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nlc OCA], [http://pdbe.org/5nlc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nlc RCSB], [http://www.ebi.ac.uk/pdbsum/5nlc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nlc ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nlc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nlc OCA], [http://pdbe.org/5nlc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nlc RCSB], [http://www.ebi.ac.uk/pdbsum/5nlc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nlc ProSAT]</span></td></tr> | ||
</table> | </table> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Anacystis nidulans r2]] | |||
[[Category: Contreras, A]] | [[Category: Contreras, A]] | ||
[[Category: Forcada-Nadal, A]] | [[Category: Forcada-Nadal, A]] | ||
Revision as of 13:19, 8 November 2017
Structure of PipY, the COG0325 family member of Synechococcus elongatus PCC7942,without PLPStructure of PipY, the COG0325 family member of Synechococcus elongatus PCC7942,without PLP
Structural highlights
Publication Abstract from PubMedThe Synechococcus elongatus COG0325 gene pipY functionally interacts with the nitrogen regulatory gene pipX. As a first step toward a molecular understanding of such interactions, we characterized PipY. This 221-residue protein is monomeric and hosts pyridoxal phosphate (PLP), binding it with limited affinity and losing it upon incubation with D-cycloserine. PipY crystal structures with and without PLP reveal a single-domain monomer folded as the TIM barrel of type-III fold PLP enzymes, with PLP highly exposed, fitting a role for PipY in PLP homeostasis. The mobile PLP phosphate-anchoring C-terminal helix might act as a trigger for PLP exchange. Exploiting the universality of COG0325 functions, we used PipY in site-directed mutagenesis studies to shed light on disease causation by epilepsy-associated mutations in the human COG0325 gene PROSC. Studies on cyanobacterial protein PipY shed light on structure, potential functions, and vitamin B6 -dependent epilepsy.,Tremino L, Forcada-Nadal A, Contreras A, Rubio V FEBS Lett. 2017 Sep 15. doi: 10.1002/1873-3468.12841. PMID:28914444[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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