5c6v: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==Crystal structure of the rice Topless related protein 2 (TPR2) N-terminal domain (1-209) in complex with Arabidopsis NINJA peptide== | ==Crystal structure of the rice Topless related protein 2 (TPR2) N-terminal domain (1-209) in complex with Arabidopsis NINJA peptide== | ||
<StructureSection load='5c6v' size='340' side='right' caption='[[5c6v]], [[Resolution|resolution]] 3.10Å' scene=''> | <StructureSection load='5c6v' size='340' side='right' caption='[[5c6v]], [[Resolution|resolution]] 3.10Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5c6v]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C6V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5C6V FirstGlance]. <br> | <table><tr><td colspan='2'>[[5c6v]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Orysa Orysa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C6V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5C6V FirstGlance]. <br> | ||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4zhe|4zhe]], [[5c7e|5c7e]], [[5c6q|5c6q]], [[5c7f|5c7f]]</td></tr> | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4zhe|4zhe]], [[5c7e|5c7e]], [[5c6q|5c6q]], [[5c7f|5c7f]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5c6v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c6v OCA], [http://pdbe.org/5c6v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5c6v RCSB], [http://www.ebi.ac.uk/pdbsum/5c6v PDBsum]</span></td></tr> | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">P0705D01.10-1, ASPR2, Os01g0254100, OsJ_01134 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4530 ORYSA])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5c6v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c6v OCA], [http://pdbe.org/5c6v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5c6v RCSB], [http://www.ebi.ac.uk/pdbsum/5c6v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5c6v ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | == Function == | ||
| Line 21: | Line 23: | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Orysa]] | |||
[[Category: Brunzelle, J S]] | [[Category: Brunzelle, J S]] | ||
[[Category: Gu, X]] | [[Category: Gu, X]] | ||
Revision as of 15:25, 6 November 2017
Structural highlights
Function[NINJA_ARATH] Acts as a transcriptional repressor. Negative regulator of jasmonate responses. Connects the JAZ proteins with the TOPLESS corepressors.[1] Publication Abstract from PubMedTOPLESS (TPL) and TOPLESS-related (TPR) proteins comprise a conserved family of plant transcriptional corepressors that are related to Tup1, Groucho, and TLE (transducin-like enhancer of split) corepressors in yeast, insects, and mammals. In plants, TPL/TPR corepressors regulate development, stress responses, and hormone signaling through interaction with small ethylene response factor-associated amphiphilic repression (EAR) motifs found in diverse transcriptional repressors. How EAR motifs can interact with TPL/TPR proteins is unknown. We confirm the amino-terminal domain of the TPL family of corepressors, which we term TOPLESS domain (TPD), as the EAR motif-binding domain. To understand the structural basis of this interaction, we determined the crystal structures of the TPD of rice (Os) TPR2 in apo (apo protein) state and in complexes with the EAR motifs from Arabidopsis NINJA (novel interactor of JAZ), IAA1 (auxin-responsive protein 1), and IAA10, key transcriptional repressors involved in jasmonate and auxin signaling. The OsTPR2 TPD adopts a new fold of nine helices, followed by a zinc finger, which are arranged into a disc-like tetramer. The EAR motifs in the three different complexes adopt a similar extended conformation with the hydrophobic residues fitting into the same surface groove of each OsTPR2 monomer. Sequence alignments and structure-based mutagenesis indicate that this mode of corepressor binding is highly conserved in a large set of transcriptional repressors, thus providing a general mechanism for gene repression mediated by the TPL family of corepressors. Structural basis for recognition of diverse transcriptional repressors by the TOPLESS family of corepressors.,Ke J, Ma H, Gu X, Thelen A, Brunzelle JS, Li J, Xu HE, Melcher K Sci Adv. 2015 Jul 24;1(6):e1500107. eCollection 2015 Jul. PMID:26601214[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
|
| ||||||||||||||||||