1nme: Difference between revisions
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==Structure of Casp-3 with tethered salicylate== | ==Structure of Casp-3 with tethered salicylate== | ||
<StructureSection load='1nme' size='340' side='right' caption='[[1nme]], [[Resolution|resolution]] 1.60Å' scene=''> | <StructureSection load='1nme' size='340' side='right' caption='[[1nme]], [[Resolution|resolution]] 1.60Å' scene=''> | ||
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=158:3-(2-MERCAPTO-ACETYLAMINO)-4-OXO-PENTANOIC+ACID'>158</scene>, <scene name='pdbligand=159:2-HYDROXY-5-(2-MERCAPTO-ETHYLSULFAMOYL)-BENZOIC+ACID'>159</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=158:3-(2-MERCAPTO-ACETYLAMINO)-4-OXO-PENTANOIC+ACID'>158</scene>, <scene name='pdbligand=159:2-HYDROXY-5-(2-MERCAPTO-ETHYLSULFAMOYL)-BENZOIC+ACID'>159</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1nmq|1nmq]], [[1nms|1nms]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1nmq|1nmq]], [[1nms|1nms]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nme FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nme OCA], [http://pdbe.org/1nme PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1nme RCSB], [http://www.ebi.ac.uk/pdbsum/1nme PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nme FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nme OCA], [http://pdbe.org/1nme PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1nme RCSB], [http://www.ebi.ac.uk/pdbsum/1nme PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1nme ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
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<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
</jmolCheckbox> | </jmolCheckbox> | ||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/ | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nme ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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</div> | </div> | ||
<div class="pdbe-citations 1nme" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 1nme" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 13:05, 1 November 2017
Structure of Casp-3 with tethered salicylateStructure of Casp-3 with tethered salicylate
Structural highlights
Function[CASP3_HUMAN] Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.[1] [2] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedCysteine aspartyl protease-3 (caspase-3) is a mediator of apoptosis and a therapeutic target for a wide range of diseases. Using a dynamic combinatorial technology, 'extended tethering', we identified unique nonpeptidic inhibitors for this enzyme. Extended tethering allowed the identification of ligands that bind to discrete regions of caspase-3 and also helped direct the assembly of these ligands into small-molecule inhibitors. We first designed a small-molecule 'extender' that irreversibly alkylates the cysteine residue of caspase-3 and also contains a thiol group. The modified protein was then screened against a library of disulfide-containing small-molecule fragments. Mass-spectrometry was used to identify ligands that bind noncovalently to the protein and that also form a disulfide linkage with the extender. Linking the selected fragments with binding elements from the extenders generates reversible, tight-binding molecules that are druglike and distinct from known inhibitors. One molecule derived from this approach inhibited apoptosis in cells. In situ assembly of enzyme inhibitors using extended tethering.,Erlanson DA, Lam JW, Wiesmann C, Luong TN, Simmons RL, DeLano WL, Choong IC, Burdett MT, Flanagan WM, Lee D, Gordon EM, O'Brien T Nat Biotechnol. 2003 Mar;21(3):308-14. Epub 2003 Feb 3. PMID:12563278[3] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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