1oio: Difference between revisions
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==GafD (F17c-type) Fimbrial adhesin from Escherichia coli== | |||
<StructureSection load='1oio' size='340' side='right' caption='[[1oio]], [[Resolution|resolution]] 1.70Å' scene=''> | <StructureSection load='1oio' size='340' side='right' caption='[[1oio]], [[Resolution|resolution]] 1.70Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1oio]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OIO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OIO FirstGlance]. <br> | <table><tr><td colspan='2'>[[1oio]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OIO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OIO FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1oio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oio OCA], [http://pdbe.org/1oio PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1oio RCSB], [http://www.ebi.ac.uk/pdbsum/1oio PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1oio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oio OCA], [http://pdbe.org/1oio PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1oio RCSB], [http://www.ebi.ac.uk/pdbsum/1oio PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1oio ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
Revision as of 12:43, 1 November 2017
GafD (F17c-type) Fimbrial adhesin from Escherichia coliGafD (F17c-type) Fimbrial adhesin from Escherichia coli
Structural highlights
Function[F17GG_ECOLX] Essential fimbrial adhesion factor that mediates binding to N-acetylglucosamine-containing receptors in the host intestinal microvilli, leading to colonization of the intestinal tissue, and diarrhea or septicemia. Also confers adhesiveness to laminin and basement membranes. May be involved in the initiation of polymerization of fimbrillin monomers during fimbrial filament biogenesis.[1] [2] Publication Abstract from PubMedGafD in Escherichia coli G (F17) fimbriae is associated with diarrheal disease, and the structure of the ligand-binding domain, GafD1-178, has been determined at 1.7A resolution in the presence of the receptor sugar N-acetyl-D-glucosamine. The overall fold is a beta-barrel jelly-roll fold. The ligand-binding site was identified and localized to the side of the molecule. Receptor binding is mediated by side-chain as well main-chain interactions. Ala43-Asn44, Ser116-Thr117 form the sugar acetamide specificity pocket, while Asp88 confers tight binding and Trp109 appears to position the ligand. There is a disulfide bond that rigidifies the acetamide specificity pocket. The three fimbrial lectins, GafD, FimH and PapG share similar beta-barrel folds but display different ligand-binding regions and disulfide-bond patterns. We suggest an evolutionary path for the evolution of the very diverse fimbrial lectins from a common ancestral fold. The structural basis of receptor-binding by Escherichia coli associated with diarrhea and septicemia.,Merckel MC, Tanskanen J, Edelman S, Westerlund-Wikstrom B, Korhonen TK, Goldman A J Mol Biol. 2003 Aug 22;331(4):897-905. PMID:12909017[3] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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