8cat: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==The NADPH binding site on beef liver catalase== | ==The NADPH binding site on beef liver catalase== | ||
<StructureSection load='8cat' size='340' side='right' caption='[[8cat]], [[Resolution|resolution]] 2.50Å' scene=''> | <StructureSection load='8cat' size='340' side='right' caption='[[8cat]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
| Line 5: | Line 6: | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr> | ||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Catalase Catalase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.6 1.11.1.6] </span></td></tr> | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Catalase Catalase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.6 1.11.1.6] </span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=8cat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8cat OCA], [http://pdbe.org/8cat PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=8cat RCSB], [http://www.ebi.ac.uk/pdbsum/8cat PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=8cat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8cat OCA], [http://pdbe.org/8cat PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=8cat RCSB], [http://www.ebi.ac.uk/pdbsum/8cat PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=8cat ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| Line 28: | Line 29: | ||
</div> | </div> | ||
<div class="pdbe-citations 8cat" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 8cat" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 12:30, 1 November 2017
The NADPH binding site on beef liver catalaseThe NADPH binding site on beef liver catalase
Structural highlights
Function[CATA_BOVIN] Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedBeef liver and human erythrocyte catalases (EC 1.11.1.6) bind NADP tenaciously [Kirkman, H. N. & Gaetani, G. F. (1984) Proc. Natl. Acad. Sci. USA 81, 4343-4348]. The position of NADP on beef liver catalase corresponds to the carboxyl-terminal polypeptide hinge in Penicillium vitale fungal catalase, which connects the common catalase structure to the additional flavodoxin-like domain. In contrast to nearly all other known structures of protein-bound NADP, NAD, and FAD, the NADP molecule of beef liver catalase is folded into a right-handed helix and bound, in part, in the vicinity of the carboxyl end of two alpha-helices. A water molecule (W7) occupies a pseudosubstrate site close to the C4 position of the nicotinamide and is hydrogen bonded to His-304. Although the NADP and heme groups approach each other to within 13.7 A, there is no direct interaction. The function of the NADP remains a mystery. The NADPH binding site on beef liver catalase.,Fita I, Rossmann MG Proc Natl Acad Sci U S A. 1985 Mar;82(6):1604-8. PMID:3856839[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References |
| ||||||||||||||||||