3l4x: Difference between revisions
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==Crystal complex of N-terminal Human Maltase-Glucoamylase with NR4-8== | ==Crystal complex of N-terminal Human Maltase-Glucoamylase with NR4-8== | ||
<StructureSection load='3l4x' size='340' side='right' caption='[[3l4x]], [[Resolution|resolution]] 1.90Å' scene=''> | <StructureSection load='3l4x' size='340' side='right' caption='[[3l4x]], [[Resolution|resolution]] 1.90Å' scene=''> | ||
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3l4t|3l4t]], [[3l4u|3l4u]], [[3l4v|3l4v]], [[3l4w|3l4w]], [[3l4y|3l4y]], [[3l4z|3l4z]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3l4t|3l4t]], [[3l4u|3l4u]], [[3l4v|3l4v]], [[3l4w|3l4w]], [[3l4y|3l4y]], [[3l4z|3l4z]]</td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MGA, MGAM, MGAML ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MGA, MGAM, MGAML ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3l4x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l4x OCA], [http://pdbe.org/3l4x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3l4x RCSB], [http://www.ebi.ac.uk/pdbsum/3l4x PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3l4x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l4x OCA], [http://pdbe.org/3l4x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3l4x RCSB], [http://www.ebi.ac.uk/pdbsum/3l4x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3l4x ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
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[[Category: Membrane]] | [[Category: Membrane]] | ||
[[Category: Multifunctional enzyme]] | [[Category: Multifunctional enzyme]] | ||
[[Category: Polymorphism]] | |||
[[Category: Signal-anchor]] | [[Category: Signal-anchor]] | ||
[[Category: Sulfation]] | [[Category: Sulfation]] | ||
[[Category: Transmembrane]] | [[Category: Transmembrane]] | ||
Revision as of 11:47, 1 November 2017
Crystal complex of N-terminal Human Maltase-Glucoamylase with NR4-8Crystal complex of N-terminal Human Maltase-Glucoamylase with NR4-8
Structural highlights
Function[MGA_HUMAN] May serve as an alternate pathway for starch digestion when luminal alpha-amylase activity is reduced because of immaturity or malnutrition. May play a unique role in the digestion of malted dietary oligosaccharides used in food manufacturing. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedAn approach to controlling blood glucose levels in individuals with type 2 diabetes is to target alpha-amylases and intestinal glucosidases using alpha-glucosidase inhibitors acarbose and miglitol. One of the intestinal glucosidases targeted is the N-terminal catalytic domain of maltase-glucoamylase (ntMGAM), one of the four intestinal glycoside hydrolase 31 enzyme activities responsible for the hydrolysis of terminal starch products into glucose. Here we present the X-ray crystallographic studies of ntMGAM in complex with a new class of alpha-glucosidase inhibitors derived from natural extracts of Salacia reticulata, a plant used traditionally in Ayuverdic medicine for the treatment of type 2 diabetes. Included in these extracts are the active compounds salacinol, kotalanol, and de-O-sulfonated kotalanol. This study reveals that de-O-sulfonated kotalanol is the most potent ntMGAM inhibitor reported to date (K(i) = 0.03 microM), some 2000-fold better than the compounds currently used in the clinic, and highlights the potential of the salacinol class of inhibitors as future drug candidates. New glucosidase inhibitors from an ayurvedic herbal treatment for type 2 diabetes: structures and inhibition of human intestinal maltase-glucoamylase with compounds from Salacia reticulata.,Sim L, Jayakanthan K, Mohan S, Nasi R, Johnston BD, Pinto BM, Rose DR Biochemistry. 2010 Jan 26;49(3):443-51. PMID:20039683[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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