1zlb: Difference between revisions
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|SITE= | |SITE= | ||
|LIGAND= | |LIGAND= | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4] </span> | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY=[[1umv|1UMV]], [[1zl7|1ZL7]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zlb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zlb OCA], [http://www.ebi.ac.uk/pdbsum/1zlb PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1zlb RCSB]</span> | |||
}} | }} | ||
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[[Category: toxin]] | [[Category: toxin]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:38:13 2008'' | ||
Revision as of 01:38, 31 March 2008
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| , resolution 0.97Å | |||||||
|---|---|---|---|---|---|---|---|
| Activity: | Phospholipase A(2), with EC number 3.1.1.4 | ||||||
| Related: | 1UMV, 1ZL7
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of catalytically-active phospholipase A2 in the absence of calcium
OverviewOverview
The electrophile Ca(2+) is an essential multifunctional co-factor in the phospholipase A(2) mediated hydrolysis of phospholipids. Crystal structures of an acidic phospholipase A(2) from the venom of Bothrops jararacussu have been determined both in the Ca(2+) free and bound states at 0.97 and 1.60 A resolutions, respectively. In the Ca(2+) bound state, the Ca(2+) ion is penta-coordinated by a distorted pyramidal cage of oxygen and nitrogen atoms that is significantly different to that observed in structures of other Group I/II phospholipases A(2). In the absence of Ca(2+), a water molecule occupies the position of the Ca(2+) ion and the side chain of Asp49 and the calcium-binding loop adopts a different conformation.
About this StructureAbout this Structure
1ZLB is a Single protein structure of sequence from Bothrops jararacussu. Full crystallographic information is available from OCA.
ReferenceReference
Insights into metal ion binding in phospholipases A2: ultra high-resolution crystal structures of an acidic phospholipase A2 in the Ca2+ free and bound states., Murakami MT, Gabdoulkhakov A, Genov N, Cintra AC, Betzel C, Arni RK, Biochimie. 2006 May;88(5):543-9. Epub 2005 Dec 5. PMID:16376474
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