1z3x: Difference between revisions
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|PDB= 1z3x |SIZE=350|CAPTION= <scene name='initialview01'>1z3x</scene>, resolution 1.50Å | |PDB= 1z3x |SIZE=350|CAPTION= <scene name='initialview01'>1z3x</scene>, resolution 1.50Å | ||
|SITE= | |SITE= | ||
|LIGAND= | |LIGAND= <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= Gun4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=146786 Thermosynechococcus elongatus]) | |GENE= Gun4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=146786 Thermosynechococcus elongatus]) | ||
|DOMAIN= | |||
|RELATEDENTRY=[[1z3y|1Z3Y]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z3x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z3x OCA], [http://www.ebi.ac.uk/pdbsum/1z3x PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1z3x RCSB]</span> | |||
}} | }} | ||
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[[Category: transferase activator]] | [[Category: transferase activator]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:28:46 2008'' | ||
Revision as of 01:28, 31 March 2008
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| , resolution 1.50Å | |||||||
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| Ligands: | |||||||
| Gene: | Gun4 (Thermosynechococcus elongatus) | ||||||
| Related: | 1Z3Y
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of Gun4 from Thermosynechococcus elongatus
OverviewOverview
Gun4 has been implicated in a developmental signaling pathway between the chloroplast and the nucleus involving magnesium protoporphyrin IX (MgP(IX)), the first dedicated intermediate in the chlorophyll biosynthetic pathway. Here we present the crystal structure of Thermosynechococcus elongatus Gun4 at 1.5 A, describe the binding affinities of Gun4 for substrate and product porphyrin molecules, and identify a likely (Mg)P(IX) binding site on the protein. Kinetic analyses show that Gun4 dramatically increases the efficiency of transformation of porphyrin substrate to metalloporphyrin product and that it also reduces the threshold Mg2+ concentration required for activity at low porphyrin concentration. Gun4 therefore controls magnesium chelatase at physiologically significant Mg2+ concentrations and likely acts as a molecular switch in vivo so that in its absence magnesium chelatase is inactive. This mechanism could allow Gun4 to mediate magnesium protoporphyrin levels both for chlorophyll biosynthesis and for signaling to the nucleus.
About this StructureAbout this Structure
1Z3X is a Single protein structure of sequence from Thermosynechococcus elongatus. Full crystallographic information is available from OCA.
ReferenceReference
Structural and biochemical characterization of Gun4 suggests a mechanism for its role in chlorophyll biosynthesis., Davison PA, Schubert HL, Reid JD, Iorg CD, Heroux A, Hill CP, Hunter CN, Biochemistry. 2005 May 31;44(21):7603-12. PMID:15909975
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