1lpw: Difference between revisions
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==Solution structure of the yeast spliceosomal U2 snRNA-intron branch site helix featuring a conserved pseudouridine== | ==Solution structure of the yeast spliceosomal U2 snRNA-intron branch site helix featuring a conserved pseudouridine== | ||
<StructureSection load='1lpw' size='340' side='right' caption='[[1lpw]], [[NMR_Ensembles_of_Models | 9 NMR models]]' scene=''> | <StructureSection load='1lpw' size='340' side='right' caption='[[1lpw]], [[NMR_Ensembles_of_Models | 9 NMR models]]' scene=''> | ||
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</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PSU:PSEUDOURIDINE-5-MONOPHOSPHATE'>PSU</scene></td></tr> | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PSU:PSEUDOURIDINE-5-MONOPHOSPHATE'>PSU</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1lmv|1lmv]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1lmv|1lmv]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lpw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lpw OCA], [http://pdbe.org/1lpw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1lpw RCSB], [http://www.ebi.ac.uk/pdbsum/1lpw PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lpw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lpw OCA], [http://pdbe.org/1lpw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1lpw RCSB], [http://www.ebi.ac.uk/pdbsum/1lpw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1lpw ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
Revision as of 13:39, 18 October 2017
Solution structure of the yeast spliceosomal U2 snRNA-intron branch site helix featuring a conserved pseudouridineSolution structure of the yeast spliceosomal U2 snRNA-intron branch site helix featuring a conserved pseudouridine
Structural highlights
Publication Abstract from PubMedPairing of a consensus sequence of the precursor (pre)-mRNA intron with a short region of the U2 small nuclear (sn)RNA during assembly of the eukaryotic spliceosome results in formation of a complementary helix of seven base pairs with a single unpaired adenosine residue. The 2' OH of this adenosine, called the branch site, brings about nucleophilic attack at the pre-mRNA 5' splice site in the first step of splicing. Another feature of this pairing is the phylogenetic conservation of a pseudouridine (psi) residue in U2 snRNA nearly opposite the branch site. We show that the presence of this psi in the pre-mRNA branch-site helix of Saccharomyces cerevisiae induces a dramatically altered architectural landscape compared with that of its unmodified counterpart. The psi-induced structure places the nucleophile in an accessible position for the first step of splicing. Sculpting of the spliceosomal branch site recognition motif by a conserved pseudouridine.,Newby MI, Greenbaum NL Nat Struct Biol. 2002 Dec;9(12):958-65. PMID:12426583[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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