2fpk: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==RadA recombinase in complex with ADP== | ==RadA recombinase in complex with ADP== | ||
<StructureSection load='2fpk' size='340' side='right' caption='[[2fpk]], [[Resolution|resolution]] 2.10Å' scene=''> | <StructureSection load='2fpk' size='340' side='right' caption='[[2fpk]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
| Line 6: | Line 7: | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2fpl|2fpl]], [[2fpm|2fpm]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2fpl|2fpl]], [[2fpm|2fpm]]</td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RADA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2188 ATCC 33273])</td></tr> | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RADA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2188 ATCC 33273])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fpk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fpk OCA], [http://pdbe.org/2fpk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2fpk RCSB], [http://www.ebi.ac.uk/pdbsum/2fpk PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fpk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fpk OCA], [http://pdbe.org/2fpk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2fpk RCSB], [http://www.ebi.ac.uk/pdbsum/2fpk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2fpk ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| Line 29: | Line 30: | ||
</div> | </div> | ||
<div class="pdbe-citations 2fpk" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 2fpk" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 10:42, 18 October 2017
RadA recombinase in complex with ADPRadA recombinase in complex with ADP
Structural highlights
Function[RADA_METVO] Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules (By similarity). Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedMembers of a superfamily of RecA-like recombinases facilitate a central strand exchange reaction in the DNA repair process. Archaeal RadA and Rad51 and eukaryal Rad51 and meiosis-specific DMC1 form a closely related group of recombinases distinct from bacterial RecA. Nevertheless, all such recombinases share a conserved core domain which carries the ATPase site and putative DNA-binding sites. Here we present the crystal structure of an archaeal RadA from Methanococcus voltae (MvRadA) in complex with ADP and Mg2+ at 2.1 A resolution. The crystallized RadA-ADP filament has an extended helical pitch similar to those of previously determined structures in the presence of nonhydrolyzable ATP analogue AMP-PNP. Structural comparison reveals two recurrent conformations with an extensive allosteric effect spanning the ATPase site and the putative DNA-binding L2 region. Varied conformations of the L2 region also imply a dynamic nature of recombinase-bound DNA. Crystal structure of Methanococcus voltae RadA in complex with ADP: hydrolysis-induced conformational change.,Qian X, Wu Y, He Y, Luo Y Biochemistry. 2005 Oct 25;44(42):13753-61. PMID:16229465[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
|
| ||||||||||||||||||||