2f96: Difference between revisions
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==2.1 A crystal structure of Pseudomonas aeruginosa rnase T (Ribonuclease T)== | ==2.1 A crystal structure of Pseudomonas aeruginosa rnase T (Ribonuclease T)== | ||
<StructureSection load='2f96' size='340' side='right' caption='[[2f96]], [[Resolution|resolution]] 2.09Å' scene=''> | <StructureSection load='2f96' size='340' side='right' caption='[[2f96]], [[Resolution|resolution]] 2.09Å' scene=''> | ||
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rnt, PA3528 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr> | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rnt, PA3528 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f96 OCA], [http://pdbe.org/2f96 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2f96 RCSB], [http://www.ebi.ac.uk/pdbsum/2f96 PDBsum], [http://www.topsan.org/Proteins/MCSG/2f96 TOPSAN]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f96 OCA], [http://pdbe.org/2f96 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2f96 RCSB], [http://www.ebi.ac.uk/pdbsum/2f96 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2f96 ProSAT], [http://www.topsan.org/Proteins/MCSG/2f96 TOPSAN]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
Revision as of 10:35, 18 October 2017
2.1 A crystal structure of Pseudomonas aeruginosa rnase T (Ribonuclease T)2.1 A crystal structure of Pseudomonas aeruginosa rnase T (Ribonuclease T)
Structural highlights
Function[RNT_PSEAE] Trims short 3' overhangs of a variety of RNA species, leaving a one or two nucleotide 3' overhang. Responsible for the end-turnover of tRNA: specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis (By similarity).[HAMAP-Rule:MF_00157] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedThe 3' processing of most bacterial precursor tRNAs involves exonucleolytic trimming to yield a mature CCA end. This step is carried out by RNase T, a member of the large DEDD family of exonucleases. We report the crystal structures of RNase T from Escherichia coli and Pseudomonas aeruginosa, which show that this enzyme adopts an opposing dimeric arrangement, with the catalytic DEDD residues from one monomer closely juxtaposed with a large basic patch on the other monomer. This arrangement suggests that RNase T has to be dimeric for substrate specificity, and agrees very well with prior site-directed mutagenesis studies. The dimeric architecture of RNase T is very similar to the arrangement seen in oligoribonuclease, another bacterial DEDD family exoribonuclease. The catalytic residues in these two enzymes are organized very similarly to the catalytic domain of the third DEDD family exoribonuclease in E. coli, RNase D, which is monomeric. Crystal structure of RNase T, an exoribonuclease involved in tRNA maturation and end turnover.,Zuo Y, Zheng H, Wang Y, Chruszcz M, Cymborowski M, Skarina T, Savchenko A, Malhotra A, Minor W Structure. 2007 Apr;15(4):417-28. PMID:17437714[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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