1ys2: Difference between revisions
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|PDB= 1ys2 |SIZE=350|CAPTION= <scene name='initialview01'>1ys2</scene>, resolution 1.50Å | |PDB= 1ys2 |SIZE=350|CAPTION= <scene name='initialview01'>1ys2</scene>, resolution 1.50Å | ||
|SITE= | |SITE= | ||
|LIGAND= | |LIGAND= <scene name='pdbligand=2HS:HEXYLPHOSPHONIC+ACID+(S)-2-METHYL-3-PHENYLPROPYL+ESTER'>2HS</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span> | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY=[[3lip|3LIP]], [[1ys1|1YS1]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ys2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ys2 OCA], [http://www.ebi.ac.uk/pdbsum/1ys2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ys2 RCSB]</span> | |||
}} | }} | ||
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[[Category: Mezzetti, A.]] | [[Category: Mezzetti, A.]] | ||
[[Category: Schrag, J D.]] | [[Category: Schrag, J D.]] | ||
[[Category: ca2++ ion]] | [[Category: ca2++ ion]] | ||
[[Category: cis peptide bond leu 234]] | [[Category: cis peptide bond leu 234]] | ||
[[Category: inhibitor hexylphosphonic acid (s) 2-methyl-3-phenylpropyl ester]] | [[Category: inhibitor hexylphosphonic acid (s) 2-methyl-3-phenylpropyl ester]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:19:57 2008'' | ||
Revision as of 01:20, 31 March 2008
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| , resolution 1.50Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Activity: | Triacylglycerol lipase, with EC number 3.1.1.3 | ||||||
| Related: | 3LIP, 1YS1
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Burkholderia cepacia lipase complexed with hexylphosphonic acid (S) 2-methyl-3-phenylpropyl ester
OverviewOverview
Synthetic chemists often exploit the high enantioselectivity of lipases to prepare pure enantiomers of primary alcohols, but the molecular basis for this enantioselectivity is unknown. The crystal structures of two phosphonate transition-state analogs bound to Burkholderia cepacia lipase reveal this molecular basis for a typical primary alcohol: 2-methyl-3-phenyl-1-propanol. The enantiomeric alcohol moieties adopt surprisingly similar orientations, with only subtle differences that make it difficult to predict how to alter enantioselectivity. These structures, along with a survey of previous structures of enzyme bound enantiomers, reveal that binding of enantiomers does not involve an exchange of two substituent positions as most researchers assumed. Instead, the enantiomers adopt mirror-image packing, where three of the four substituents at the stereocenter lie in similar positions. The fourth substituent, hydrogen, points in opposite directions.
About this StructureAbout this Structure
1YS2 is a Single protein structure of sequence from Burkholderia cepacia. Full crystallographic information is available from OCA.
ReferenceReference
Mirror-image packing in enantiomer discrimination molecular basis for the enantioselectivity of B.cepacia lipase toward 2-methyl-3-phenyl-1-propanol., Mezzetti A, Schrag JD, Cheong CS, Kazlauskas RJ, Chem Biol. 2005 Apr;12(4):427-37. PMID:15850979
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