2zc6: Difference between revisions
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==Penicillin-binding protein 1A (PBP 1A) acyl-enzyme complex (tebipenem) from Streptococcus pneumoniae== | ==Penicillin-binding protein 1A (PBP 1A) acyl-enzyme complex (tebipenem) from Streptococcus pneumoniae== | ||
<StructureSection load='2zc6' size='340' side='right' caption='[[2zc6]], [[Resolution|resolution]] 2.70Å' scene=''> | <StructureSection load='2zc6' size='340' side='right' caption='[[2zc6]], [[Resolution|resolution]] 2.70Å' scene=''> | ||
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TEB:(4R,5S)-3-(1-(4,5-DIHYDROTHIAZOL-2-YL)AZETIDIN-3-YLTHIO)-5-((2S,3R)-3-HYDROXY-1-OXOBUTAN-2-YL)-4-METHYL-4,5-+DIHYDRO-1H-PYRROLE-2-CARBOXYLIC+ACID'>TEB</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TEB:(4R,5S)-3-(1-(4,5-DIHYDROTHIAZOL-2-YL)AZETIDIN-3-YLTHIO)-5-((2S,3R)-3-HYDROXY-1-OXOBUTAN-2-YL)-4-METHYL-4,5-+DIHYDRO-1H-PYRROLE-2-CARBOXYLIC+ACID'>TEB</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2zc3|2zc3]], [[2zc4|2zc4]], [[2zc5|2zc5]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2zc3|2zc3]], [[2zc4|2zc4]], [[2zc5|2zc5]]</td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pbpA, exp2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pbpA, exp2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= Streptococcus pneumoniae]), pbp1a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= Streptococcus pneumoniae])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zc6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zc6 OCA], [http://pdbe.org/2zc6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2zc6 RCSB], [http://www.ebi.ac.uk/pdbsum/2zc6 PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zc6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zc6 OCA], [http://pdbe.org/2zc6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2zc6 RCSB], [http://www.ebi.ac.uk/pdbsum/2zc6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2zc6 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
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</div> | </div> | ||
<div class="pdbe-citations 2zc6" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 2zc6" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 17:43, 12 October 2017
Penicillin-binding protein 1A (PBP 1A) acyl-enzyme complex (tebipenem) from Streptococcus pneumoniaePenicillin-binding protein 1A (PBP 1A) acyl-enzyme complex (tebipenem) from Streptococcus pneumoniae
Structural highlights
Function[PBPA_STRR6] Cell wall formation. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedBiapenem is a parenteral carbapenem antibiotic that exhibits wide-ranging antibacterial activity, remarkable chemical stability, and extensive stability against human renal dehydropeptidase-I. Tebipenem is the active form of tebipenem pivoxil, a novel oral carbapenem antibiotic that has a high level of bioavailability in humans, in addition to the above-mentioned features. beta-lactam antibiotics, including carbapenems, target penicillin-binding proteins (PBPs), which are membrane-associated enzymes that play essential roles in peptidoglycan biosynthesis. To envisage the binding of carbapenems to PBPs, we determined the crystal structures of the trypsin-digested forms of both PBP 2X and PBP 1A from Streptococcus pneumoniae strain R6, each complexed with biapenem or tebipenem. The structures of the complexes revealed that the carbapenem C-2 side chains form hydrophobic interactions with Trp374 and Thr526 of PBP 2X and with Trp411 and Thr543 of PBP 1A. The Trp and Thr residues are conserved in PBP 2B. These results suggest that interactions between the C-2 side chains of carbapenems and the conserved Trp and Thr residues in PBPs play important roles in the binding of carbapenems to PBPs. Crystal structures of biapenem and tebipenem complexed with penicillin-binding proteins 2X and 1A from Streptococcus pneumoniae.,Yamada M, Watanabe T, Baba N, Takeuchi Y, Ohsawa F, Gomi S Antimicrob Agents Chemother. 2008 Jun;52(6):2053-60. Epub 2008 Apr 7. PMID:18391040[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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