1xmt: Difference between revisions
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==X-ray structure of gene product from arabidopsis thaliana at1g77540== | ==X-ray structure of gene product from arabidopsis thaliana at1g77540== | ||
<StructureSection load='1xmt' size='340' side='right' caption='[[1xmt]], [[Resolution|resolution]] 1.15Å' scene=''> | <StructureSection load='1xmt' size='340' side='right' caption='[[1xmt]], [[Resolution|resolution]] 1.15Å' scene=''> | ||
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">At1g77540 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr> | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">At1g77540 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xmt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xmt OCA], [http://pdbe.org/1xmt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xmt RCSB], [http://www.ebi.ac.uk/pdbsum/1xmt PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xmt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xmt OCA], [http://pdbe.org/1xmt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xmt RCSB], [http://www.ebi.ac.uk/pdbsum/1xmt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1xmt ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
Revision as of 11:09, 12 October 2017
X-ray structure of gene product from arabidopsis thaliana at1g77540X-ray structure of gene product from arabidopsis thaliana at1g77540
Structural highlights
Function[Y1754_ARATH] Possesses in vitro histone acetyltransferase activity with histones H3 and H4.[1] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedWe describe X-ray crystal and NMR solution structures of the protein coded for by Arabidopsis thaliana gene At1g77540.1 (At1g77540). The crystal structure was determined to 1.15 A with an R factor of 14.9% (Rfree = 17.0%) by multiple-wavelength anomalous diffraction using sodium bromide derivatized crystals. The ensemble of NMR conformers was determined with protein samples labeled with 15N and 13C + 15N. The X-ray structure and NMR ensemble were closely similar with rmsd 1.4 A for residues 8-93. At1g77540 was found to adopt a fold similar to that of GCN5-related N-acetyltransferases. Enzymatic activity assays established that At1g77540 possesses weak acetyltransferase activity against histones H3 and H4. Chemical shift perturbations observed in 15N-HSQC spectra upon the addition of CoA indicated that the cofactor binds and identified its binding site. The molecular details of this interaction were further elucidated by solving the X-ray structure of the At1g77540-CoA complex. This work establishes that the domain family COG2388 represents a novel class of acetyltransferase and provides insight into possible mechanistic roles of the conserved Cys76 and His41 residues of this family. Structure of Arabidopsis thaliana At1g77540 protein, a minimal acetyltransferase from the COG2388 family.,Tyler RC, Bitto E, Berndsen CE, Bingman CA, Singh S, Lee MS, Wesenberg GE, Denu JM, Phillips GN Jr, Markley JL Biochemistry. 2006 Dec 5;45(48):14325-36. PMID:17128971[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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