5ls0: Difference between revisions
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The | ==Crystal structure of Inorganic Pyrophosphatase PPA1 from Arabidopsis thaliana== | ||
<StructureSection load='5ls0' size='340' side='right' caption='[[5ls0]], [[Resolution|resolution]] 1.83Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5ls0]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LS0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LS0 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4lug|4lug]]</td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Inorganic_diphosphatase Inorganic diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.1 3.6.1.1] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ls0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ls0 OCA], [http://pdbe.org/5ls0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ls0 RCSB], [http://www.ebi.ac.uk/pdbsum/5ls0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ls0 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/IPYR1_ARATH IPYR1_ARATH]] Catalyzes the irreversible hydrolysis of pyrophosphate (PPi) to phosphate. The MgPPi(2-) complex binds to the enzyme only after a free Mg(2+) ion has bound (Ref.9). No activity with glycerol-3-phosphate, glucose-6-phosphate, p-nitrophenylphosphate, ADP, NADP(+), NAD(+),NADH, NADPH or phosphoribosyl pyrophosphate as substrates (Ref.9). Controls the equilibrium of gluconeogenic reactions in the heterotrophic growth phase of early seedling establishment. Determinates the rate of cytosolic glycolysis, providing carbon for seed storage lipid accumulation (PubMed:22566496).<ref>PMID:22566496</ref> [REFERENCE:9] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Inorganic pyrophosphatase (PPase) is a ubiquitous cytosolic enzyme which catalyzes the hydrolysis of inorganic pyrophosphate (PPi) to orthophosphate (Pi). The crystal structure of inorganic pyrophosphatase from Helicobacter pylori (H-PPase) has been solved by MAD and refined to an R factor of 20.6% at 2.6 A resolution. The crystallographic asymmetric unit contains a homohexameric H-PPase arranged as a dimer of trimers. While most of the structural elements of PPases are highly conserved in H-PPase, some unique structural features are localized in the flexible loops near the active site, suggesting that the structural flexibility of these loops is required for the catalytic efficiency of PPase. | |||
Structure of inorganic pyrophosphatase from Helicobacter pylori.,Wu CA, Lokanath NK, Kim DY, Park HJ, Hwang HY, Kim ST, Suh SW, Kim KK Acta Crystallogr D Biol Crystallogr. 2005 Nov;61(Pt 11):1459-64. Epub 2005, Oct 19. PMID:16239722<ref>PMID:16239722</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 5ls0" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Inorganic diphosphatase]] | |||
[[Category: Grzechowiak, M]] | [[Category: Grzechowiak, M]] | ||
[[Category: Jaskolski, M]] | |||
[[Category: Sikorski, M]] | [[Category: Sikorski, M]] | ||
[[Category: | [[Category: Hydrolase]] | ||
[[Category: Inorganic pyrophosphatase]] | |||
[[Category: Ob-fold]] | |||
Revision as of 08:19, 6 October 2017
Crystal structure of Inorganic Pyrophosphatase PPA1 from Arabidopsis thalianaCrystal structure of Inorganic Pyrophosphatase PPA1 from Arabidopsis thaliana
Structural highlights
Function[IPYR1_ARATH] Catalyzes the irreversible hydrolysis of pyrophosphate (PPi) to phosphate. The MgPPi(2-) complex binds to the enzyme only after a free Mg(2+) ion has bound (Ref.9). No activity with glycerol-3-phosphate, glucose-6-phosphate, p-nitrophenylphosphate, ADP, NADP(+), NAD(+),NADH, NADPH or phosphoribosyl pyrophosphate as substrates (Ref.9). Controls the equilibrium of gluconeogenic reactions in the heterotrophic growth phase of early seedling establishment. Determinates the rate of cytosolic glycolysis, providing carbon for seed storage lipid accumulation (PubMed:22566496).[1] [REFERENCE:9] Publication Abstract from PubMedInorganic pyrophosphatase (PPase) is a ubiquitous cytosolic enzyme which catalyzes the hydrolysis of inorganic pyrophosphate (PPi) to orthophosphate (Pi). The crystal structure of inorganic pyrophosphatase from Helicobacter pylori (H-PPase) has been solved by MAD and refined to an R factor of 20.6% at 2.6 A resolution. The crystallographic asymmetric unit contains a homohexameric H-PPase arranged as a dimer of trimers. While most of the structural elements of PPases are highly conserved in H-PPase, some unique structural features are localized in the flexible loops near the active site, suggesting that the structural flexibility of these loops is required for the catalytic efficiency of PPase. Structure of inorganic pyrophosphatase from Helicobacter pylori.,Wu CA, Lokanath NK, Kim DY, Park HJ, Hwang HY, Kim ST, Suh SW, Kim KK Acta Crystallogr D Biol Crystallogr. 2005 Nov;61(Pt 11):1459-64. Epub 2005, Oct 19. PMID:16239722[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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