Purine repressor: Difference between revisions

Revision as of 01:29, 22 September 2017

Function

Purine repressor (PurR) is a member of the lac repressor family. PurP binds DNA via a highly conserved helix-turn-helix at the N terminal (DBD). PurP contains a nucleotide co-repressor binding domain as well (CBD). PurP binds to a 16-bp operator sequence and co-regulates genes which are involved in the biosynthesis of purine and pyrimidine nucleotides^[1].

Structural highlights

The . Water molecules shown as red spheres. The DNA binding domain contains a and a hinge helix binding to to the DNA minor groove. ^[2].

Structure of E. coli purine processor complex with DNA and guanine (PDB entry 1wet)

Drag the structure with the mouse to rotate

3D structures of purine repressor3D structures of purine repressor

Updated on 22-September-2017

ReferencesReferences

↑ Wilson HR, Turnbough CL Jr. Role of the purine repressor in the regulation of pyrimidine gene expression in Escherichia coli K-12. J Bacteriol. 1990 Jun;172(6):3208-13. PMID:1971621
↑ Schumacher MA, Glasfeld A, Zalkin H, Brennan RG. The X-ray structure of the PurR-guanine-purF operator complex reveals the contributions of complementary electrostatic surfaces and a water-mediated hydrogen bond to corepressor specificity and binding affinity. J Biol Chem. 1997 Sep 5;272(36):22648-53. PMID:9278422

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Joel L. Sussman

[1] Wilson HR, Turnbough CL Jr. Role of the purine repressor in the regulation of pyrimidine gene expression in Escherichia coli K-12. J Bacteriol. 1990 Jun;172(6):3208-13. PMID:1971621

[2] Schumacher MA, Glasfeld A, Zalkin H, Brennan RG. The X-ray structure of the PurR-guanine-purF operator complex reveals the contributions of complementary electrostatic surfaces and a water-mediated hydrogen bond to corepressor specificity and binding affinity. J Biol Chem. 1997 Sep 5;272(36):22648-53. PMID:9278422

[1]

[2]

@@ Line 1: / Line 1: @@
-<StructureSection load='1wet' size='450' side='right' caption='Structure of E. coli purine processor complex with DNA and guanine (PDB entry [[1wet]])' scene='55/554904/Cv/1'>
+<StructureSection load='' size='450' side='right' caption='Structure of E. coli purine processor complex with DNA and guanine (PDB entry [[1wet]])' scene='55/554904/Cv/1'>
 == Function ==
 '''Purine repressor''' (PurR) is a member of the lac repressor family.  PurP binds DNA via a highly conserved helix-turn-helix at the N terminal (DBD).  PurP contains a nucleotide co-repressor binding domain as well (CBD).  PurP binds to a 16-bp operator sequence and co-regulates genes which are involved in the biosynthesis of purine and pyrimidine nucleotides<ref>PMID:1971621</ref>.