5tw5: Difference between revisions
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==Structure of mouse CD1d with bound glycosphingolipid JJ112== | |||
<StructureSection load='5tw5' size='340' side='right' caption='[[5tw5]], [[Resolution|resolution]] 1.85Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5tw5]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TW5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TW5 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=7LM:N-[(2S,3S,4R)-1-(alpha-D-galactopyranosyloxy)-3,4-dihydroxy-16-phenylhexadecan-2-yl]octanamide'>7LM</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5tw2|5tw2]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5tw5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tw5 OCA], [http://pdbe.org/5tw5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5tw5 RCSB], [http://www.ebi.ac.uk/pdbsum/5tw5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5tw5 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/CD1D1_MOUSE CD1D1_MOUSE]] Antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on natural killer T-cells.<ref>PMID:11754812</ref> <ref>PMID:16314439</ref> <ref>PMID:16007091</ref> [[http://www.uniprot.org/uniprot/B2MG_MOUSE B2MG_MOUSE]] Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Invariant Natural Killer T-cells (iNKT-cells) are an attractive target for immune response modulation, as upon CD1d-mediated stimulation with KRN7000, a synthetic alpha-galactosylceramide, they produce a vast amount of cytokines. Here we present a synthesis that allows swift modification of the phytosphingosine side chain by amidation of an advanced methyl ester precursor. The resulting KRN7000 derivatives, termed alpha-galactosylsphingamides, were evaluated for their capacity to stimulate iNKT-cells. While introduction of the amide-motif in the phytosphingosine chain is tolerated for CD1d binding and TCR recognition, the studied alpha-galactosylsphingamides showed compromised antigenic properties. | |||
Galactosylsphingamides: new alpha-GalCer analogues to probe the F'-pocket of CD1d.,Guillaume J, Wang J, Janssens J, Remesh SG, Risseeuw MDP, Decruy T, Froeyen M, Elewaut D, Zajonc DM, Calenbergh SV Sci Rep. 2017 Jun 27;7(1):4276. doi: 10.1038/s41598-017-04461-7. PMID:28655912<ref>PMID:28655912</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 5tw5" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Wang, J]] | [[Category: Wang, J]] | ||
[[Category: Zajonc, D M]] | |||
[[Category: Antigen-presentation]] | |||
[[Category: Glycolipid]] | |||
[[Category: Immune system-inhibitor complex]] | |||
[[Category: Mhc fold]] | |||
[[Category: T cell]] | |||
Revision as of 07:33, 21 September 2017
Structure of mouse CD1d with bound glycosphingolipid JJ112Structure of mouse CD1d with bound glycosphingolipid JJ112
Structural highlights
Function[CD1D1_MOUSE] Antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on natural killer T-cells.[1] [2] [3] [B2MG_MOUSE] Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Publication Abstract from PubMedInvariant Natural Killer T-cells (iNKT-cells) are an attractive target for immune response modulation, as upon CD1d-mediated stimulation with KRN7000, a synthetic alpha-galactosylceramide, they produce a vast amount of cytokines. Here we present a synthesis that allows swift modification of the phytosphingosine side chain by amidation of an advanced methyl ester precursor. The resulting KRN7000 derivatives, termed alpha-galactosylsphingamides, were evaluated for their capacity to stimulate iNKT-cells. While introduction of the amide-motif in the phytosphingosine chain is tolerated for CD1d binding and TCR recognition, the studied alpha-galactosylsphingamides showed compromised antigenic properties. Galactosylsphingamides: new alpha-GalCer analogues to probe the F'-pocket of CD1d.,Guillaume J, Wang J, Janssens J, Remesh SG, Risseeuw MDP, Decruy T, Froeyen M, Elewaut D, Zajonc DM, Calenbergh SV Sci Rep. 2017 Jun 27;7(1):4276. doi: 10.1038/s41598-017-04461-7. PMID:28655912[4] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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