1hk5: Difference between revisions
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Revision as of 17:32, 5 November 2007
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HUMAN SERUM ALBUMIN MUTANT R218H COMPLEXED WITH THYROXINE (3,3',5,5'-TETRAIODO-L-THYRONINE) AND MYRISTIC ACID (TETRADECANOIC ACID)
OverviewOverview
Human serum albumin (HSA) is the major protein component of blood plasma, and serves as a transporter for thyroxine and other hydrophobic compounds, such as fatty acids and bilirubin. We report here a structural, characterization of HSA-thyroxine interactions. Using crystallographic, analyses we have identified four binding sites for thyroxine on HSA, distributed in subdomains IIA, IIIA, and IIIB. Mutation of residue R218, within subdomain IIA greatly enhances the affinity for thyroxine and, causes the elevated serum thyroxine levels associated with familial, dysalbuminemic hyperthyroxinemia (FDH). Structural analysis of two FDH, mutants of HSA (R218H and R218P) shows that this effect arises because, substitution of R218, which contacts the hormone bound in subdomain IIA, produces localized conformational changes to relax steric restrictions on, thyroxine binding at this site. We have also found that, although fatty, acid binding competes with thyroxine at all four sites, it induces, conformational changes that create a fifth hormone-binding site in the, cleft between domains I and III, at least 9 A from R218. These structural, observations are consistent with binding data showing that HSA retains a, high-affinity site for thyroxine in the presence of excess fatty acid that, is insensitive to FDH mutations.
About this StructureAbout this Structure
1HK5 is a Single protein structure of sequence from Homo sapiens with MYR and T44 as ligands. Structure known Active Site: AC1. Full crystallographic information is available from OCA.
ReferenceReference
Structural basis of albumin-thyroxine interactions and familial dysalbuminemic hyperthyroxinemia., Petitpas I, Petersen CE, Ha CE, Bhattacharya AA, Zunszain PA, Ghuman J, Bhagavan NV, Curry S, Proc Natl Acad Sci U S A. 2003 May 27;100(11):6440-5. Epub 2003 May 12. PMID:12743361
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