5u3e: Difference between revisions
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==Crystal Structure of Native Lectin from Canavalia bonariensis Seeds (CaBo) complexed with alpha-methyl-D-mannoside== | |||
<StructureSection load='5u3e' size='340' side='right' caption='[[5u3e]], [[Resolution|resolution]] 2.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5u3e]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Canavalia_bonariensis Canavalia bonariensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U3E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5U3E FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MMA:O1-METHYL-MANNOSE'>MMA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5u3e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u3e OCA], [http://pdbe.org/5u3e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5u3e RCSB], [http://www.ebi.ac.uk/pdbsum/5u3e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5u3e ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
CaBo is a mannose/glucose-specific lectin purified from seeds of Canavalia bonariensis. In the present work, we report the CaBo crystal structure determined to atomic resolution in the presence of X-man, a specific ligand. Similar to the structural characteristics of other legume lectins, CaBo presented the jellyroll motif, a metal binding site occupied by calcium and manganese ions close to the carbohydrate-recognition domain (CRD). In vitro test of CaBo cytotoxicity against glioma cells demonstrated its ability to decrease the cellular viability and migration by induction of autophagy and cell death. Molecular docking simulations corroborate previous data indicating that the lectin's biological activities occur mostly through interactions with glycoproteins since the lectin interacted favorably with several N-glycans, especially those of the high-mannose type. Together, these results suggest that CaBo interacts with glycosylated cell targets and elicits a remarkable antiglioma activity. | |||
Canavalia bonariensis lectin: Molecular bases of glycoconjugates interaction and antiglioma potential.,Cavada BS, Silva MTL, Osterne VJS, Pinto-Junior VR, Nascimento APM, Wolin IAV, Heinrich IA, Nobre CAS, Moreira CG, Lossio CF, Rocha CRC, Martins JL, Nascimento KS, Leal RB Int J Biol Macromol. 2017 Aug 10. pii: S0141-8130(17)32351-6. doi:, 10.1016/j.ijbiomac.2017.08.023. PMID:28803976<ref>PMID:28803976</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5u3e" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: | [[Category: Canavalia bonariensis]] | ||
[[Category: Osterne, V | [[Category: Araripe, D A]] | ||
[[Category: | [[Category: Cavada, B S]] | ||
[[Category: | [[Category: Leal, R B]] | ||
[[Category: | [[Category: Martins, J L]] | ||
[[Category: Silva, M | [[Category: Nascimento, K S]] | ||
[[Category: | [[Category: Neco, A H.B]] | ||
[[Category: Osterne, V J.S]] | |||
[[Category: Pinto-Junior, V R]] | |||
[[Category: Rocha, C R.C]] | |||
[[Category: Santiago, M Q]] | |||
[[Category: Silva, M T.L]] | |||
[[Category: Silva-Filho, J C]] | |||
[[Category: Alpha-methyl-d-mannoside]] | |||
[[Category: Lectin]] | |||
[[Category: Sugar binding protein]] | |||
Revision as of 07:08, 30 August 2017
Structural highlights
Publication Abstract from PubMedCaBo is a mannose/glucose-specific lectin purified from seeds of Canavalia bonariensis. In the present work, we report the CaBo crystal structure determined to atomic resolution in the presence of X-man, a specific ligand. Similar to the structural characteristics of other legume lectins, CaBo presented the jellyroll motif, a metal binding site occupied by calcium and manganese ions close to the carbohydrate-recognition domain (CRD). In vitro test of CaBo cytotoxicity against glioma cells demonstrated its ability to decrease the cellular viability and migration by induction of autophagy and cell death. Molecular docking simulations corroborate previous data indicating that the lectin's biological activities occur mostly through interactions with glycoproteins since the lectin interacted favorably with several N-glycans, especially those of the high-mannose type. Together, these results suggest that CaBo interacts with glycosylated cell targets and elicits a remarkable antiglioma activity. Canavalia bonariensis lectin: Molecular bases of glycoconjugates interaction and antiglioma potential.,Cavada BS, Silva MTL, Osterne VJS, Pinto-Junior VR, Nascimento APM, Wolin IAV, Heinrich IA, Nobre CAS, Moreira CG, Lossio CF, Rocha CRC, Martins JL, Nascimento KS, Leal RB Int J Biol Macromol. 2017 Aug 10. pii: S0141-8130(17)32351-6. doi:, 10.1016/j.ijbiomac.2017.08.023. PMID:28803976[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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