1dp3: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==SOLUTION STRUCTURE OF THE DNA BINDING DOMAIN OF THE TRAM PROTEIN== | ==SOLUTION STRUCTURE OF THE DNA BINDING DOMAIN OF THE TRAM PROTEIN== | ||
<StructureSection load='1dp3' size='340' side='right' caption='[[1dp3]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | <StructureSection load='1dp3' size='340' side='right' caption='[[1dp3]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1dp3]] is a 1 chain structure | <table><tr><td colspan='2'>[[1dp3]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DP3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DP3 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dp3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dp3 OCA], [http://pdbe.org/1dp3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1dp3 RCSB], [http://www.ebi.ac.uk/pdbsum/1dp3 PDBsum]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dp3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dp3 OCA], [http://pdbe.org/1dp3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1dp3 RCSB], [http://www.ebi.ac.uk/pdbsum/1dp3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1dp3 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| Line 15: | Line 16: | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
</jmolCheckbox> | </jmolCheckbox> | ||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/ | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dp3 ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
| Line 30: | Line 31: | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Bermel, W]] | [[Category: Bermel, W]] | ||
[[Category: Hoegenauer, G]] | [[Category: Hoegenauer, G]] | ||
Revision as of 07:06, 30 August 2017
SOLUTION STRUCTURE OF THE DNA BINDING DOMAIN OF THE TRAM PROTEINSOLUTION STRUCTURE OF THE DNA BINDING DOMAIN OF THE TRAM PROTEIN
Structural highlights
Function[TRAM2_ECOLX] Conjugative DNA transfer (CDT) is the unidirectional transfer of ssDNA plasmid from a donor to a recipient cell. It is the central mechanism by which antibiotic resistance and virulence factors are propagated in bacterial populations. Part of the relaxosome, which facilitates a site- and strand-specific cut in the origin of transfer by TraI, at the nic site. Probably autoregulates its own expression. Plasmid specificity is conferred by the TraD-TraM pair (By similarity). Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedThe solution structure of the DNA-binding domain of the TraM protein, an essential component of the DNA transfer machinery of the conjugative resistance plasmid R1, is presented. The structure has been determined using homonuclear 2-dimensional NMR spectroscopy as well as 15N labeled heteronuclear 2- and 3-dimensional NMR spectroscopy. It turns out that the solution structure of the DNA binding domain of the TraM protein is globular and dominantly helical. The very first amino acids of the N-terminus are unstructured. Solution structure of the DNA-binding domain of TraM.,Stockner T, Plugariu C, Koraimann G, Hogenauer G, Bermel W, Prytulla S, Sterk H Biochemistry. 2001 Mar 20;40(11):3370-7. PMID:11258958[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References |
| ||||||||||||||