5o09: Difference between revisions

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'''Unreleased structure'''


The entry 5o09 is ON HOLD
==BtubABC mini microtubule==
<StructureSection load='5o09' size='340' side='right' caption='[[5o09]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5o09]] is a 24 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O09 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5O09 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5o01|5o01]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5o09 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o09 OCA], [http://pdbe.org/5o09 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5o09 RCSB], [http://www.ebi.ac.uk/pdbsum/5o09 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5o09 ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Microtubules, the dynamic, yet stiff hollow tubes built from alphabeta-tubulin protein heterodimers, are thought to be present only in eukaryotic cells. Here, we report a 3.6-A helical reconstruction electron cryomicroscopy structure of four-stranded mini microtubules formed by bacterial tubulin-like Prosthecobacter dejongeii BtubAB proteins. Despite their much smaller diameter, mini microtubules share many key structural features with eukaryotic microtubules, such as an M-loop, alternating subunits, and a seam that breaks overall helical symmetry. Using in vitro total internal reflection fluorescence microscopy, we show that bacterial mini microtubules treadmill and display dynamic instability, another hallmark of eukaryotic microtubules. The third protein in the btub gene cluster, BtubC, previously known as "bacterial kinesin light chain," binds along protofilaments every 8 nm, inhibits BtubAB mini microtubule catastrophe, and increases rescue. Our work reveals that some bacteria contain regulated and dynamic cytomotive microtubule systems that were once thought to be only useful in much larger and sophisticated eukaryotic cells.


Authors: Deng, X., Bharat, T.A.M., Lowe, J.
Four-stranded mini microtubules formed by Prosthecobacter BtubAB show dynamic instability.,Deng X, Fink G, Bharat TAM, He S, Kureisaite-Ciziene D, Lowe J Proc Natl Acad Sci U S A. 2017 Jul 18;114(29):E5950-E5958. doi:, 10.1073/pnas.1705062114. Epub 2017 Jul 3. PMID:28673988<ref>PMID:28673988</ref>


Description: BtubABC mini microtubule
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
<div class="pdbe-citations 5o09" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bharat, T A.M]]
[[Category: Deng, X]]
[[Category: Lowe, J]]
[[Category: Lowe, J]]
[[Category: Deng, X]]
[[Category: Bacterial cytoskeleton]]
[[Category: Bharat, T.A.M]]
[[Category: Microtubule]]
[[Category: Structural protein]]

Revision as of 06:56, 30 August 2017

BtubABC mini microtubuleBtubABC mini microtubule

Structural highlights

5o09 is a 24 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

Microtubules, the dynamic, yet stiff hollow tubes built from alphabeta-tubulin protein heterodimers, are thought to be present only in eukaryotic cells. Here, we report a 3.6-A helical reconstruction electron cryomicroscopy structure of four-stranded mini microtubules formed by bacterial tubulin-like Prosthecobacter dejongeii BtubAB proteins. Despite their much smaller diameter, mini microtubules share many key structural features with eukaryotic microtubules, such as an M-loop, alternating subunits, and a seam that breaks overall helical symmetry. Using in vitro total internal reflection fluorescence microscopy, we show that bacterial mini microtubules treadmill and display dynamic instability, another hallmark of eukaryotic microtubules. The third protein in the btub gene cluster, BtubC, previously known as "bacterial kinesin light chain," binds along protofilaments every 8 nm, inhibits BtubAB mini microtubule catastrophe, and increases rescue. Our work reveals that some bacteria contain regulated and dynamic cytomotive microtubule systems that were once thought to be only useful in much larger and sophisticated eukaryotic cells.

Four-stranded mini microtubules formed by Prosthecobacter BtubAB show dynamic instability.,Deng X, Fink G, Bharat TAM, He S, Kureisaite-Ciziene D, Lowe J Proc Natl Acad Sci U S A. 2017 Jul 18;114(29):E5950-E5958. doi:, 10.1073/pnas.1705062114. Epub 2017 Jul 3. PMID:28673988[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

  1. Deng X, Fink G, Bharat TAM, He S, Kureisaite-Ciziene D, Lowe J. Four-stranded mini microtubules formed by Prosthecobacter BtubAB show dynamic instability. Proc Natl Acad Sci U S A. 2017 Jul 18;114(29):E5950-E5958. doi:, 10.1073/pnas.1705062114. Epub 2017 Jul 3. PMID:28673988 doi:http://dx.doi.org/10.1073/pnas.1705062114

5o09, resolution 3.60Å

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OCA