181d: Difference between revisions
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==SEQUENCE-DEPENDENT MICROHETEROGENEITY OF Z-DNA: THE CRYSTAL AND MOLECULAR STRUCTURES OF D(CACGCG).D(CGCGTG) AND D(CGCACG).D(CGTGCG)== | ==SEQUENCE-DEPENDENT MICROHETEROGENEITY OF Z-DNA: THE CRYSTAL AND MOLECULAR STRUCTURES OF D(CACGCG).D(CGCGTG) AND D(CGCACG).D(CGTGCG)== | ||
<StructureSection load='181d' size='340' side='right' caption='[[181d]], [[Resolution|resolution]] 1.60Å' scene=''> | <StructureSection load='181d' size='340' side='right' caption='[[181d]], [[Resolution|resolution]] 1.60Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[181d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=181D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=181D FirstGlance]. <br> | <table><tr><td colspan='2'>[[181d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=181D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=181D FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=181d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=181d OCA], [http://pdbe.org/181d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=181d RCSB], [http://www.ebi.ac.uk/pdbsum/181d PDBsum]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=181d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=181d OCA], [http://pdbe.org/181d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=181d RCSB], [http://www.ebi.ac.uk/pdbsum/181d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=181d ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
Revision as of 12:10, 9 August 2017
SEQUENCE-DEPENDENT MICROHETEROGENEITY OF Z-DNA: THE CRYSTAL AND MOLECULAR STRUCTURES OF D(CACGCG).D(CGCGTG) AND D(CGCACG).D(CGTGCG)SEQUENCE-DEPENDENT MICROHETEROGENEITY OF Z-DNA: THE CRYSTAL AND MOLECULAR STRUCTURES OF D(CACGCG).D(CGCGTG) AND D(CGCACG).D(CGTGCG)
Structural highlights
Publication Abstract from PubMedWe have solved and refined the crystal structures of the first two non-self-complementary hexadeoxyribonucleotide duplexes with Watson-Crick base pairs, namely d(CACGCG).d(CGCGTG) and d(CGCACG).d(CGTGCG). Both the hexamers crystallize in the left-handed Z-DNA conformation. The packing of the molecules is similar in the two crystals: the hexamers are stacked on top of each other to form columns of infinite length, which are arranged in a close-packed hexagonal pattern. In spite of the similar packing, crystals of the first duplex belong to the space group P2(1)2(1)2(1), isomorphous to previous Z-DNA hexamers, while crystals of the other duplex belong to the space group P2(1), which has not been observed before in this context. The molecular structures of the two duplexes are also different. The first is very similar in conformation to the other Z-DNA hexamers and to the idealized ZI model. The other duplex shows large differences in the stacking and the relative disposition of its bases, leading to its possible classification as a novel Z-DNA variant. We conclude that the differences in the structure under similar environmental conditions point to a sequence-dependent plasticity in the DNA molecule which is visible even under close-packed conditions. Sequence-dependent microheterogeneity of Z-DNA: the crystal and molecular structures of d(CACGCG).d(CGCGTG) and d(CGCACG).d(CGTGCG).,Sadasivan C, Gautham N J Mol Biol. 1995 May 19;248(5):918-30. PMID:7760333[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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