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'''Unreleased structure'''


The entry 5mqw is ON HOLD  until Paper Publication
==High-speed fixed-target serial virus crystallography==
<StructureSection load='5mqw' size='340' side='right' caption='[[5mqw]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5mqw]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MQW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MQW FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mqw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mqw OCA], [http://pdbe.org/5mqw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mqw RCSB], [http://www.ebi.ac.uk/pdbsum/5mqw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mqw ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We report a method for serial X-ray crystallography at X-ray free-electron lasers (XFELs), which allows for full use of the current 120-Hz repetition rate of the Linear Coherent Light Source (LCLS). Using a micropatterned silicon chip in combination with the high-speed Roadrunner goniometer for sample delivery, we were able to determine the crystal structures of the picornavirus bovine enterovirus 2 (BEV2) and the cytoplasmic polyhedrosis virus type 18 polyhedrin, with total data collection times of less than 14 and 10 min, respectively. Our method requires only micrograms of sample and should therefore broaden the applicability of serial femtosecond crystallography to challenging projects for which only limited sample amounts are available. By synchronizing the sample exchange to the XFEL repetition rate, our method allows for most efficient use of the limited beam time available at XFELs and should enable a substantial increase in sample throughput at these facilities.


Authors: Roedig, P., Ginn, H.M., Pakendorf, T., Sutton, G., Harlos, K., Walter, T.S., Meyer, J., Fischer, P., Duman, R., Vartiainen, I., Reime, B., Warmer, M., Brewster, A.S., Young, I.D., Michels-Clark, T., Sauter, N.K., Sikorsky, M., Nelson, S., Damiani, D.S., Alonso-Mori, R., Ren, J., Fry, E.E., David, C., Sturt, D.I., Wagner, A., Meents, A.
High-speed fixed-target serial virus crystallography.,Roedig P, Ginn HM, Pakendorf T, Sutton G, Harlos K, Walter TS, Meyer J, Fischer P, Duman R, Vartiainen I, Reime B, Warmer M, Brewster AS, Young ID, Michels-Clark T, Sauter NK, Kotecha A, Kelly J, Rowlands DJ, Sikorsky M, Nelson S, Damiani DS, Alonso-Mori R, Ren J, Fry EE, David C, Stuart DI, Wagner A, Meents A Nat Methods. 2017 Aug;14(8):805-810. doi: 10.1038/nmeth.4335. Epub 2017 Jun 19. PMID:28628129<ref>PMID:28628129</ref>


Description: High-speed fixed-target serial virus crystallography
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
<div class="pdbe-citations 5mqw" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Alonso-Mori, R]]
[[Category: Brewster, A S]]
[[Category: Damiani, D S]]
[[Category: David, C]]
[[Category: Duman, R]]
[[Category: Duman, R]]
[[Category: Walter, T.S]]
[[Category: Fischer, P]]
[[Category: Ren, J]]
[[Category: Fry, E E]]
[[Category: Sutton, G]]
[[Category: Ginn, H M]]
[[Category: Harlos, K]]
[[Category: Meents, A]]
[[Category: Meyer, J]]
[[Category: Michels-Clark, T]]
[[Category: Michels-Clark, T]]
[[Category: Wagner, A]]
[[Category: Warmer, M]]
[[Category: Meyer, J]]
[[Category: Damiani, D.S]]
[[Category: Nelson, S]]
[[Category: Nelson, S]]
[[Category: Pakendorf, T]]
[[Category: Pakendorf, T]]
[[Category: Harlos, K]]
[[Category: Reime, B]]
[[Category: Ren, J]]
[[Category: Roedig, P]]
[[Category: Sauter, N K]]
[[Category: Sikorsky, M]]
[[Category: Sikorsky, M]]
[[Category: Sauter, N.K]]
[[Category: Stuart, D I]]
[[Category: Young, I.D]]
[[Category: Sutton, G]]
[[Category: Sturt, D.I]]
[[Category: Brewster, A.S]]
[[Category: Ginn, H.M]]
[[Category: Fischer, P]]
[[Category: Meents, A]]
[[Category: Vartiainen, I]]
[[Category: Vartiainen, I]]
[[Category: Fry, E.E]]
[[Category: Wagner, A]]
[[Category: Reime, B]]
[[Category: Walter, T S]]
[[Category: Roedig, P]]
[[Category: Warmer, M]]
[[Category: David, C]]
[[Category: Young, I D]]
[[Category: Alonso-Mori, R]]
[[Category: Cpv18]]
[[Category: Cypovirus polyhedrin]]
[[Category: Viral protein]]

Revision as of 14:32, 3 August 2017

High-speed fixed-target serial virus crystallographyHigh-speed fixed-target serial virus crystallography

Structural highlights

5mqw is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:, , ,
NonStd Res:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

We report a method for serial X-ray crystallography at X-ray free-electron lasers (XFELs), which allows for full use of the current 120-Hz repetition rate of the Linear Coherent Light Source (LCLS). Using a micropatterned silicon chip in combination with the high-speed Roadrunner goniometer for sample delivery, we were able to determine the crystal structures of the picornavirus bovine enterovirus 2 (BEV2) and the cytoplasmic polyhedrosis virus type 18 polyhedrin, with total data collection times of less than 14 and 10 min, respectively. Our method requires only micrograms of sample and should therefore broaden the applicability of serial femtosecond crystallography to challenging projects for which only limited sample amounts are available. By synchronizing the sample exchange to the XFEL repetition rate, our method allows for most efficient use of the limited beam time available at XFELs and should enable a substantial increase in sample throughput at these facilities.

High-speed fixed-target serial virus crystallography.,Roedig P, Ginn HM, Pakendorf T, Sutton G, Harlos K, Walter TS, Meyer J, Fischer P, Duman R, Vartiainen I, Reime B, Warmer M, Brewster AS, Young ID, Michels-Clark T, Sauter NK, Kotecha A, Kelly J, Rowlands DJ, Sikorsky M, Nelson S, Damiani DS, Alonso-Mori R, Ren J, Fry EE, David C, Stuart DI, Wagner A, Meents A Nat Methods. 2017 Aug;14(8):805-810. doi: 10.1038/nmeth.4335. Epub 2017 Jun 19. PMID:28628129[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

  1. ↑ Roedig P, Ginn HM, Pakendorf T, Sutton G, Harlos K, Walter TS, Meyer J, Fischer P, Duman R, Vartiainen I, Reime B, Warmer M, Brewster AS, Young ID, Michels-Clark T, Sauter NK, Kotecha A, Kelly J, Rowlands DJ, Sikorsky M, Nelson S, Damiani DS, Alonso-Mori R, Ren J, Fry EE, David C, Stuart DI, Wagner A, Meents A. High-speed fixed-target serial virus crystallography. Nat Methods. 2017 Aug;14(8):805-810. doi: 10.1038/nmeth.4335. Epub 2017 Jun 19. PMID:28628129 doi:http://dx.doi.org/10.1038/nmeth.4335

5mqw, resolution 2.40Å

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