5ul5: Difference between revisions

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'''Unreleased structure'''


The entry 5ul5 is ON HOLD  until Paper Publication
==Crystal structure of RPE65 in complex with MB-004 and palmitate==
 
<StructureSection load='5ul5' size='340' side='right' caption='[[5ul5]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
Authors: Kiser, P.D., Palczewski, K.
== Structural highlights ==
 
<table><tr><td colspan='2'>[[5ul5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UL5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UL5 FirstGlance]. <br>
Description: Crystal structure of RPE65 in complex with MB-004 and palmitate
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=8D7:(1R)-3-AMINO-1-{3-[(2-PROPYLPENTYL)OXY]PHENYL}PROPAN-1-OL'>8D7</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PG6:1-(2-METHOXY-ETHOXY)-2-{2-[2-(2-METHOXY-ETHOXY]-ETHOXY}-ETHANE'>PG6</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>
[[Category: Unreleased Structures]]
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5ulg|5ulg]]</td></tr>
[[Category: Kiser, P.D]]
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Retinoid_isomerohydrolase Retinoid isomerohydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.64 3.1.1.64] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ul5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ul5 OCA], [http://pdbe.org/5ul5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ul5 RCSB], [http://www.ebi.ac.uk/pdbsum/5ul5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ul5 ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/RPE65_BOVIN RPE65_BOVIN]] Plays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. The enzymatic activity is linearly dependent of the expression levels and membrane association.<ref>PMID:16096063</ref> <ref>PMID:19805034</ref> <ref>PMID:20100834</ref> 
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bos taurus]]
[[Category: Retinoid isomerohydrolase]]
[[Category: Kiser, P D]]
[[Category: Palczewski, K]]
[[Category: Palczewski, K]]
[[Category: Inhibitor]]
[[Category: Isomerase]]
[[Category: Isomerase-isomerase inhibitor complex]]
[[Category: Non-heme iron enzyme]]
[[Category: Retinoid isomerase]]

Revision as of 18:50, 17 May 2017

Crystal structure of RPE65 in complex with MB-004 and palmitateCrystal structure of RPE65 in complex with MB-004 and palmitate

Structural highlights

5ul5 is a 2 chain structure with sequence from Bos taurus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:, , , ,
Activity:Retinoid isomerohydrolase, with EC number 3.1.1.64
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RPE65_BOVIN] Plays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. The enzymatic activity is linearly dependent of the expression levels and membrane association.[1] [2] [3]

References

  1. ↑ Jin M, Li S, Moghrabi WN, Sun H, Travis GH. Rpe65 is the retinoid isomerase in bovine retinal pigment epithelium. Cell. 2005 Aug 12;122(3):449-59. PMID:16096063 doi:10.1016/j.cell.2005.06.042
  2. ↑ Kiser PD, Golczak M, Lodowski DT, Chance MR, Palczewski K. Crystal structure of native RPE65, the retinoid isomerase of the visual cycle. Proc Natl Acad Sci U S A. 2009 Oct 13;106(41):17325-30. Epub 2009 Oct 5. PMID:19805034
  3. ↑ Golczak M, Kiser PD, Lodowski DT, Maeda A, Palczewski K. Importance of membrane structural integrity for RPE65 retinoid isomerization activity. J Biol Chem. 2010 Mar 26;285(13):9667-82. Epub 2010 Jan 25. PMID:20100834 doi:10.1074/jbc.M109.063941

5ul5, resolution 2.20Å

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