4rai: Difference between revisions
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==Crystal Structure of CNG mimicking NaK-ETPP mutant in complex with Na+== | ==Crystal Structure of CNG mimicking NaK-ETPP mutant in complex with Na+== | ||
<StructureSection load='4rai' size='340' side='right' caption='[[4rai]], [[Resolution|resolution]] 2.31Å' scene=''> | <StructureSection load='4rai' size='340' side='right' caption='[[4rai]], [[Resolution|resolution]] 2.31Å' scene=''> | ||
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLY:GLYCINE'>GLY</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLY:GLYCINE'>GLY</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3k0d|3k0d]], [[3k0g|3k0g]], [[4r50|4r50]], [[4r6z|4r6z]], [[4r7c|4r7c]], [[4r8b|4r8b]], [[4r8c|4r8c]], [[4rar|4rar]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3k0d|3k0d]], [[3k0g|3k0g]], [[4r50|4r50]], [[4r6z|4r6z]], [[4r7c|4r7c]], [[4r8b|4r8b]], [[4r8c|4r8c]], [[4rar|4rar]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rai FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rai OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rai RCSB], [http://www.ebi.ac.uk/pdbsum/4rai PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rai FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rai OCA], [http://pdbe.org/4rai PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rai RCSB], [http://www.ebi.ac.uk/pdbsum/4rai PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rai ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4rai" style="background-color:#fffaf0;"></div> | |||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 17:19, 27 April 2017
Crystal Structure of CNG mimicking NaK-ETPP mutant in complex with Na+Crystal Structure of CNG mimicking NaK-ETPP mutant in complex with Na+
Structural highlights
Publication Abstract from PubMedCyclic nucleotide-gated (CNG) ion channels, despite a significant homology with the highly selective K+ channels, do not discriminate among monovalent alkali cations and are permeable also to several organic cations. We combined electrophysiology, molecular dynamics (MD) simulations, and X-ray crystallography to demonstrate that the pore of CNG channels is highly flexible. When a CNG mimic is crystallized in the presence of a variety of monovalent cations, including Na+, Cs+, and dimethylammonium (DMA+), the side chain of Glu66 in the selectivity filter shows multiple conformations and the diameter of the pore changes significantly. MD simulations indicate that Glu66 and the prolines in the outer vestibule undergo large fluctuations, which are modulated by the ionic species and the voltage. This flexibility underlies the coupling between gating and permeation and the poor ionic selectivity of CNG channels. A structural, functional, and computational analysis suggests pore flexibility as the base for the poor selectivity of CNG channels.,Napolitano LM, Bisha I, De March M, Marchesi A, Arcangeletti M, Demitri N, Mazzolini M, Rodriguez A, Magistrato A, Onesti S, Laio A, Torre V Proc Natl Acad Sci U S A. 2015 Jun 22. pii: 201503334. PMID:26100907[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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