5aey: Difference between revisions
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==actin-like ParM protein bound to AMPPNP== | ==actin-like ParM protein bound to AMPPNP== | ||
<StructureSection load='5aey' size='340' side='right' caption='[[5aey]], [[Resolution|resolution]] 4.30Å' scene=''> | <StructureSection load='5aey' size='340' side='right' caption='[[5aey]], [[Resolution|resolution]] 4.30Å' scene=''> | ||
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<table><tr><td colspan='2'>[[5aey]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AEY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AEY FirstGlance]. <br> | <table><tr><td colspan='2'>[[5aey]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AEY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AEY FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5aey FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5aey OCA], [http://www.rcsb.org/pdb/explore.do?structureId=5aey RCSB], [http://www.ebi.ac.uk/pdbsum/5aey PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5aey FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5aey OCA], [http://pdbe.org/5aey PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5aey RCSB], [http://www.ebi.ac.uk/pdbsum/5aey PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5aey ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 5aey" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Plasmid segregation protein ParM|Plasmid segregation protein ParM]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 16:28, 19 April 2017
actin-like ParM protein bound to AMPPNPactin-like ParM protein bound to AMPPNP
Structural highlights
Function[PARM_ECOLX] Involved in the control of plasmid partition. Required for the accurate segregation of the plasmid. Publication Abstract from PubMedActive segregation of Escherichia coli low-copy-number plasmid R1 involves formation of a bipolar spindle made of left-handed double-helical actin-like ParM filaments. ParR links the filaments with centromeric parC plasmid DNA, while facilitating the addition of subunits to ParM filaments. Growing ParMRC spindles push sister plasmids to the cell poles. Here, using modern electron cryomicroscopy methods, we investigate the structures and arrangements of ParM filaments in vitro and in cells, revealing at near-atomic resolution how subunits and filaments come together to produce the simplest known mitotic machinery. To understand the mechanism of dynamic instability, we determine structures of ParM filaments in different nucleotide states. The structure of filaments bound to the ATP analogue AMPPNP is determined at 4.3 A resolution and refined. The ParM filament structure shows strong longitudinal interfaces and weaker lateral interactions. Also using electron cryomicroscopy, we reconstruct ParM doublets forming antiparallel spindles. Finally, with whole-cell electron cryotomography, we show that doublets are abundant in bacterial cells containing low-copy-number plasmids with the ParMRC locus, leading to an asynchronous model of R1 plasmid segregation. Structures of actin-like ParM filaments show architecture of plasmid-segregating spindles.,Bharat TA, Murshudov GN, Sachse C, Lowe J Nature. 2015 Apr 27:1-5. doi: 10.1038/nature14356. PMID:25915019[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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