4fsb: Difference between revisions
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==Crystal structure of the metallo-beta-lactamase VIM-31 in its oxidized form at 1.88 A== | ==Crystal structure of the metallo-beta-lactamase VIM-31 in its oxidized form at 1.88 A== | ||
<StructureSection load='4fsb' size='340' side='right' caption='[[4fsb]], [[Resolution|resolution]] 1.88Å' scene=''> | <StructureSection load='4fsb' size='340' side='right' caption='[[4fsb]], [[Resolution|resolution]] 1.88Å' scene=''> | ||
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4fr7|4fr7]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4fr7|4fr7]]</td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">blaVIM-31 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=550 "Aerobacter cloacae" (Jordan 1890) Bergey et al. 1923])</td></tr> | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">blaVIM-31 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=550 "Aerobacter cloacae" (Jordan 1890) Bergey et al. 1923])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fsb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fsb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fsb RCSB], [http://www.ebi.ac.uk/pdbsum/4fsb PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fsb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fsb OCA], [http://pdbe.org/4fsb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4fsb RCSB], [http://www.ebi.ac.uk/pdbsum/4fsb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4fsb ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4fsb" style="background-color:#fffaf0;"></div> | |||
==See Also== | ==See Also== | ||
Revision as of 15:10, 12 April 2017
Crystal structure of the metallo-beta-lactamase VIM-31 in its oxidized form at 1.88 ACrystal structure of the metallo-beta-lactamase VIM-31 in its oxidized form at 1.88 A
Structural highlights
Publication Abstract from PubMedThe metallo-beta-lactamase VIM-31 differs from VIM-2 by only two Tyr224His and His252Arg substitutions. Located close to the active site, the Tyr224His substitution is also present in VIM-1, VIM-4, VIM-7 and VIM-12. The VIM-31 variant was reported in 2012 from Enterobacter cloacae and kinetically characterized. It exhibits globally lower catalytic efficiencies than VIM-2. In the present study, we report the three-dimensional structures of VIM-31 in its native (reduced) and oxidized forms. The so-called 'flapping-loop' (loop 1) and loop 3 of VIM-31 were not positioned as in VIM-2 but instead were closer to the active site as in VIM-4, resulting in a narrower active site in VIM-31. Also, the presence of His224 in VIM-31 disrupts hydrogen-bonding networks close to the active site. Moreover, a third zinc-binding site, which also exists in VIM-2 structures, could be identified as a structural explanation for the decreased activity of VIM-MBLs at high zinc concentrations. The three-dimensional structure of VIM-31 - a metallo-beta-lactamase from Enterobacter cloacae in its native and oxidized form.,Kupper MB, Herzog K, Bennink S, Schlomer P, Bogaerts P, Glupczynski Y, Fischer R, Bebrone C, Hoffmann KM FEBS J. 2015 Mar 30. doi: 10.1111/febs.13283. PMID:25825035[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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