4wyh: Difference between revisions
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==Crystal structure of PriX from the hyperthermophilic archaeon Sulfolobus solfataricus== | ==Crystal structure of PriX from the hyperthermophilic archaeon Sulfolobus solfataricus== | ||
<StructureSection load='4wyh' size='340' side='right' caption='[[4wyh]], [[Resolution|resolution]] 1.95Å' scene=''> | <StructureSection load='4wyh' size='340' side='right' caption='[[4wyh]], [[Resolution|resolution]] 1.95Å' scene=''> | ||
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<table><tr><td colspan='2'>[[4wyh]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WYH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WYH FirstGlance]. <br> | <table><tr><td colspan='2'>[[4wyh]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WYH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WYH FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wyh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wyh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wyh RCSB], [http://www.ebi.ac.uk/pdbsum/4wyh PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wyh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wyh OCA], [http://pdbe.org/4wyh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wyh RCSB], [http://www.ebi.ac.uk/pdbsum/4wyh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wyh ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4wyh" style="background-color:#fffaf0;"></div> | |||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 15:07, 12 April 2017
Crystal structure of PriX from the hyperthermophilic archaeon Sulfolobus solfataricusCrystal structure of PriX from the hyperthermophilic archaeon Sulfolobus solfataricus
Structural highlights
Publication Abstract from PubMedArchaea encode a eukaryotic-type primase comprising a catalytic subunit (PriS) and a noncatalytic subunit (PriL). Here we report the identification of a primase noncatalytic subunit, denoted PriX, from the hyperthermophilic archaeon Sulfolobus solfataricus. Like PriL, PriX is essential for the survival of the organism. The crystallographic analysis complemented by sensitive sequence comparisons shows that PriX is a diverged homologue of the C-terminal domain of PriL but lacks the iron-sulfur cluster. Phylogenomic analysis provides clues on the origin and evolution of PriX. PriX, PriL and PriS form a stable heterotrimer (PriSLX). Both PriSX and PriSLX show far greater affinity for nucleotide substrates and are substantially more active in primer synthesis than the PriSL heterodimer. In addition, PriL, but not PriX, facilitates primer extension by PriS. We propose that the catalytic activity of PriS is modulated through concerted interactions with the two noncatalytic subunits in primer synthesis. A primase subunit essential for efficient primer synthesis by an archaeal eukaryotic-type primase.,Liu B, Ouyang S, Makarova KS, Xia Q, Zhu Y, Li Z, Guo L, Koonin EV, Liu ZJ, Huang L Nat Commun. 2015 Jun 22;6:7300. doi: 10.1038/ncomms8300. PMID:26095544[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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