Sandbox Reserved 1066: Difference between revisions
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==Mechanism of Transport== | ==Mechanism of Transport== | ||
YiiP's ability to export Zn<sup>2+</sup> from the cytoplasm is best described as an alternating access mechanism with Zn<sup>2+</sup>/H<sup>+</sup> antiport. YiiP has 2 major structural conformations which determines what. 3H90 is a crystal structure YiiP in its outward-facing conformation where Zn<sup>2+</sup> is released from site A and H<sup>+</sup> binds in its place. 3J1Z(a Yiip homolog derived from Shewanella oneidensis) has been crystallized using cryoelectron microscopy to show an inward facing conformation. In this conformation H<sup>+</sup> releases from Site A and Zn<sup>2+</sup> binds in its place. The energy for inducing the conformation change is postulated to come from the binding energy of each substrate. The binding of Zn<sup>2+</sup> favors the outward-facing conformation and the binding of H<sup>+</sup> favors the inward-facing conformation. The proton motive force provides the driving force to export Zn<sup>2+</sup> from the cytoplasm. | |||
===Zn Induced Conformation Change=== | ===Zn Induced Conformation Change=== | ||