Sandbox Reserved 1066: Difference between revisions
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Kyle Colston (talk | contribs) No edit summary |
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==Structure== | ==Structure== | ||
YiiP is a homodimer with transmembrane (TMD) and C-terminal (CTD) domains that are connected via a charge interlocking mechanism located on a flexible loop. There are 3 Zn<sup>2+</sup> | |||
binding sites per unit of homodimer. Site A is located in the TMD, site C is located in the CTD, and site B is located at the junction of the domains join. Both TMD are composed of 6 helices, 4 of which (TM1,TM2,TM4,TM5) form a pore in which Zn<sup>2+</sup> and H<sup>+</sup> can reach binding Site A. Zn<sup>2+</sup> binding at site C helps hold the CTD together and is thought to stabilize conformational changes in YiiP. | |||
==Mechanism of Transport== | |||
===Zn Induced Conformation Change=== | ===Zn Induced Conformation Change=== | ||
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Zn binding to Active Site C causes a conformation change that reduces the affinity for Zn at Active Site A. | Zn binding to Active Site C causes a conformation change that reduces the affinity for Zn at Active Site A. | ||
== Structural highlights == | == Structural highlights == | ||