Sandbox Reserved 1052: Difference between revisions
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<Structure load='2KJB' size='350' frame='true' align='left' caption='3D Representation of CzrA with Zn Bound' scene='Insert optional scene name here' /> | <Structure load='2KJB' size='350' frame='true' align='left' caption='3D Representation of CzrA with Zn Bound' scene='Insert optional scene name here' /> | ||
==CzrA== | ==CzrA== | ||
== Biological Function == | == Biological Function == | ||
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== DNA Bound State == | == DNA Bound State == | ||
The <scene name='69/694219/Serandhisresidues/2'>main DNA interactions</scene> have been found to occur at the | The <scene name='69/694219/Serandhisresidues/2'>main DNA interactions</scene> have been found to occur at the Ser 54 and 57 along with His 58. These residues are likely to interact with the 5'-TGAA sequence found in the half-site of the DNA. These residues are found in the N terminal of the R helix. The residues involved in the <scene name='69/694219/Dna_binding_pocket/1'>DNA binding pocket</scene> are Val 42 and Gln 53. This was experimentally determined by replacing the Gln and Val with Ala residues and measuring the binding capacity; the Ka decresed by 11 and 160-fold respectively<ref name="LoC">[Arunkumar A., Campanello G., Giedroc D. (2009). Solution Structure of a | ||
paradigm ArsR family zinc sensor in the DNA-bound state. PNAS 106:43 | |||
18177-18182.]</ref> The previously mentioned Ser and His residues were found to bind the DNA with a similar affinity as the fully inhibited Zn<sup>2+</sup>. | |||
== Zinc Ligand(s) == | == Zinc Ligand(s) == | ||
== Other Ligands == | == Other Ligands == | ||
</StructureSection> | </StructureSection> | ||
== References == | == References == | ||
Arunkumar A., Campanello G., Giedroc D. (2009). Solution Structure of a | |||
paradigm ArsR family zinc sensor in the DNA-bound state. PNAS 106:43 | |||
18177-18182 | |||
Revision as of 16:30, 14 March 2017
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CzrACzrA
Biological FunctionBiological Function
Structural OverviewStructural Overview
DNA Bound StateDNA Bound State
The have been found to occur at the Ser 54 and 57 along with His 58. These residues are likely to interact with the 5'-TGAA sequence found in the half-site of the DNA. These residues are found in the N terminal of the R helix. The residues involved in the are Val 42 and Gln 53. This was experimentally determined by replacing the Gln and Val with Ala residues and measuring the binding capacity; the Ka decresed by 11 and 160-fold respectively[1] The previously mentioned Ser and His residues were found to bind the DNA with a similar affinity as the fully inhibited Zn2+.
Zinc Ligand(s)Zinc Ligand(s)
Other LigandsOther Ligands
</StructureSection>
ReferencesReferences
Arunkumar A., Campanello G., Giedroc D. (2009). Solution Structure of a paradigm ArsR family zinc sensor in the DNA-bound state. PNAS 106:43 18177-18182