5u70: Difference between revisions
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The | ==Chicken Slo2.2 in an open conformation vitrified in the presence of 300 mM NaCl== | ||
<StructureSection load='5u70' size='340' side='right' caption='[[5u70]], [[Resolution|resolution]] 3.70Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5u70]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U70 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5U70 FirstGlance]. <br> | |||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5u76|5u76]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5u70 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u70 OCA], [http://pdbe.org/5u70 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5u70 RCSB], [http://www.ebi.ac.uk/pdbsum/5u70 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5u70 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/KCNT1_CHICK KCNT1_CHICK]] Generates outwardly rectifying currents that are suppressed by elevation of intracellular calcium. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The stable structural conformations that occur along the complete reaction coordinate for ion channel opening have never been observed. In this study, we describe the equilibrium ensemble of structures of Slo2.2, a neuronal Na+-activated K+ channel, as a function of the Na+ concentration. We find that Slo2.2 exists in multiple closed conformations whose relative occupancies are independent of Na+ concentration. An open conformation emerges from an ensemble of closed conformations in a highly Na+-dependent manner, without evidence of Na+-dependent intermediates. In other words, channel opening is a highly concerted, switch-like process. The midpoint of the structural titration matches that of the functional titration. A maximum open conformation probability approaching 1.0 and maximum functional open probability approaching 0.7 imply that, within the class of open channels, there is a subclass that is not permeable to ions. | |||
Structural Titration of Slo2.2, a Na+-Dependent K+ Channel.,Hite RK, MacKinnon R Cell. 2017 Jan 26;168(3):390-399.e11. doi: 10.1016/j.cell.2016.12.030. Epub 2017 , Jan 19. PMID:28111072<ref>PMID:28111072</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5u70" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Hite, R K]] | |||
[[Category: MacKinnon, R]] | |||
[[Category: Ion channel]] | |||
[[Category: Potassium channel]] | |||
[[Category: Transport protein]] | |||
Revision as of 12:29, 10 March 2017
Chicken Slo2.2 in an open conformation vitrified in the presence of 300 mM NaClChicken Slo2.2 in an open conformation vitrified in the presence of 300 mM NaCl
Structural highlights
Function[KCNT1_CHICK] Generates outwardly rectifying currents that are suppressed by elevation of intracellular calcium. Publication Abstract from PubMedThe stable structural conformations that occur along the complete reaction coordinate for ion channel opening have never been observed. In this study, we describe the equilibrium ensemble of structures of Slo2.2, a neuronal Na+-activated K+ channel, as a function of the Na+ concentration. We find that Slo2.2 exists in multiple closed conformations whose relative occupancies are independent of Na+ concentration. An open conformation emerges from an ensemble of closed conformations in a highly Na+-dependent manner, without evidence of Na+-dependent intermediates. In other words, channel opening is a highly concerted, switch-like process. The midpoint of the structural titration matches that of the functional titration. A maximum open conformation probability approaching 1.0 and maximum functional open probability approaching 0.7 imply that, within the class of open channels, there is a subclass that is not permeable to ions. Structural Titration of Slo2.2, a Na+-Dependent K+ Channel.,Hite RK, MacKinnon R Cell. 2017 Jan 26;168(3):390-399.e11. doi: 10.1016/j.cell.2016.12.030. Epub 2017 , Jan 19. PMID:28111072[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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