4d6h: Difference between revisions
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==Crystal structure of a family 98 glycoside hydrolase catalytic module (Sp3GH98) in complex with the type 1 blood group A-tetrasaccharide (E558A X02 mutant)== | ==Crystal structure of a family 98 glycoside hydrolase catalytic module (Sp3GH98) in complex with the type 1 blood group A-tetrasaccharide (E558A X02 mutant)== | ||
<StructureSection load='4d6h' size='340' side='right' caption='[[4d6h]], [[Resolution|resolution]] 1.65Å' scene=''> | <StructureSection load='4d6h' size='340' side='right' caption='[[4d6h]], [[Resolution|resolution]] 1.65Å' scene=''> | ||
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4d6c|4d6c]], [[4d6d|4d6d]], [[4d6e|4d6e]], [[4d6f|4d6f]], [[4d6g|4d6g]], [[4d6i|4d6i]], [[4d6j|4d6j]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4d6c|4d6c]], [[4d6d|4d6d]], [[4d6e|4d6e]], [[4d6f|4d6f]], [[4d6g|4d6g]], [[4d6i|4d6i]], [[4d6j|4d6j]]</td></tr> | ||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Blood-group-substance_endo-1,4-beta-galactosidase Blood-group-substance endo-1,4-beta-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.102 3.2.1.102] </span></td></tr> | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Blood-group-substance_endo-1,4-beta-galactosidase Blood-group-substance endo-1,4-beta-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.102 3.2.1.102] </span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4d6h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d6h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4d6h RCSB], [http://www.ebi.ac.uk/pdbsum/4d6h PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4d6h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d6h OCA], [http://pdbe.org/4d6h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4d6h RCSB], [http://www.ebi.ac.uk/pdbsum/4d6h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4d6h ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4d6h" style="background-color:#fffaf0;"></div> | |||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 20:27, 9 March 2017
Crystal structure of a family 98 glycoside hydrolase catalytic module (Sp3GH98) in complex with the type 1 blood group A-tetrasaccharide (E558A X02 mutant)Crystal structure of a family 98 glycoside hydrolase catalytic module (Sp3GH98) in complex with the type 1 blood group A-tetrasaccharide (E558A X02 mutant)
Structural highlights
Publication Abstract from PubMedBlood transfusions are critically important in many medical procedures, but the presence of antigens on red blood cells (RBCs, erythrocytes) means that careful blood-typing must be carried out prior to transfusion to avoid adverse and sometimes fatal reactions following transfusion. Enzymatic removal of the terminal N-acetylgalactosamine or galactose of A- or B-antigens, respectively, yields universal O-type blood, but is inefficient. Starting with the family 98 glycoside hydrolase from Streptococcus pneumoniae SP3-BS71 (Sp3GH98), which cleaves the entire terminal trisaccharide antigenic determinants of both A- and B-antigens from some of the linkages on RBC surface glycans, through several rounds of evolution, we developed variants with vastly improved activity toward some of the linkages that are resistant to cleavage by the wild-type enzyme. The resulting enzyme effects more complete removal of blood group antigens from cell surfaces, demonstrating the potential for engineering enzymes to generate antigen-null blood from donors of various types. Toward Efficient Enzymes for the Generation of Universal Blood through Structure-Guided Directed Evolution.,Kwan DH, Constantinescu I, Chapanian R, Higgins MA, Kotzler MP, Samain E, Boraston AB, Kizhakkedathu JN, Withers SG J Am Chem Soc. 2015 Apr 24. PMID:25870881[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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