4dxm: Difference between revisions
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==Crystal Structure of an ancestral GFP-like protein== | ==Crystal Structure of an ancestral GFP-like protein== | ||
<StructureSection load='4dxm' size='340' side='right' caption='[[4dxm]], [[Resolution|resolution]] 1.40Å' scene=''> | <StructureSection load='4dxm' size='340' side='right' caption='[[4dxm]], [[Resolution|resolution]] 1.40Å' scene=''> | ||
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CR8:2-[1-AMINO-2-(1H-IMIDAZOL-5-YL)ETHYL]-1-(CARBOXYMETHYL)-4-[(4-OXOCYCLOHEXA-2,5-DIEN-1-YLIDENE)METHYL]-1H-IMIDAZOL-5-OLATE'>CR8</scene></td></tr> | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CR8:2-[1-AMINO-2-(1H-IMIDAZOL-5-YL)ETHYL]-1-(CARBOXYMETHYL)-4-[(4-OXOCYCLOHEXA-2,5-DIEN-1-YLIDENE)METHYL]-1H-IMIDAZOL-5-OLATE'>CR8</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4dxi|4dxi]], [[4dxn|4dxn]], [[4dxo|4dxo]], [[4dxp|4dxp]], [[4dxq|4dxq]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4dxi|4dxi]], [[4dxn|4dxn]], [[4dxo|4dxo]], [[4dxp|4dxp]], [[4dxq|4dxq]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dxm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dxm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4dxm RCSB], [http://www.ebi.ac.uk/pdbsum/4dxm PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dxm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dxm OCA], [http://pdbe.org/4dxm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4dxm RCSB], [http://www.ebi.ac.uk/pdbsum/4dxm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4dxm ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4dxm" style="background-color:#fffaf0;"></div> | |||
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== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 21:41, 1 February 2017
Crystal Structure of an ancestral GFP-like proteinCrystal Structure of an ancestral GFP-like protein
Structural highlights
Publication Abstract from PubMedIn proteins, functional divergence involves mutations that modify structure and dynamics. Here we provide experimental evidence for an evolutionary mechanism driven solely by long-range dynamic motions without significant backbone adjustments, catalytic group rearrangements, or changes in subunit assembly. Crystallographic structures were determined for several reconstructed ancestral proteins belonging to a GFP class frequently employed in superresolution microscopy. Their chain flexibility was analyzed using molecular dynamics and perturbation response scanning. The green-to-red photoconvertible phenotype appears to have arisen from a common green ancestor by migration of a knob-like anchoring region away from the active site diagonally across the beta barrel fold. The allosterically coupled mutational sites provide active site conformational mobility via epistasis. We propose that light-induced chromophore twisting is enhanced in a reverse-protonated subpopulation, activating internal acid-base chemistry and backbone cleavage to enlarge the chromophore. Dynamics-driven hinge migration may represent a more general platform for the evolution of novel enzyme activities. A hinge migration mechanism unlocks the evolution of green-to-red photoconversion in GFP-like proteins.,Kim H, Zou T, Modi C, Dorner K, Grunkemeyer TJ, Chen L, Fromme R, Matz MV, Ozkan SB, Wachter RM Structure. 2015 Jan 6;23(1):34-43. doi: 10.1016/j.str.2014.11.011. PMID:25565105[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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