1s7z: Difference between revisions
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|PDB= 1s7z |SIZE=350|CAPTION= <scene name='initialview01'>1s7z</scene>, resolution 1.83Å | |PDB= 1s7z |SIZE=350|CAPTION= <scene name='initialview01'>1s7z</scene>, resolution 1.83Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=CS:CESIUM ION'>CS</scene> | |LIGAND= <scene name='pdbligand=CS:CESIUM+ION'>CS</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= 0.3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= | |GENE= 0.3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10760 Enterobacteria phage T7]) | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s7z OCA], [http://www.ebi.ac.uk/pdbsum/1s7z PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1s7z RCSB]</span> | |||
}} | }} | ||
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==About this Structure== | ==About this Structure== | ||
1S7Z is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ | 1S7Z is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S7Z OCA]. | ||
==Reference== | ==Reference== | ||
Structure of Ocr from bacteriophage T7, a protein that mimics B-form DNA., Walkinshaw MD, Taylor P, Sturrock SS, Atanasiu C, Berge T, Henderson RM, Edwardson JM, Dryden DT, Mol Cell. 2002 Jan;9(1):187-94. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11804597 11804597] | Structure of Ocr from bacteriophage T7, a protein that mimics B-form DNA., Walkinshaw MD, Taylor P, Sturrock SS, Atanasiu C, Berge T, Henderson RM, Edwardson JM, Dryden DT, Mol Cell. 2002 Jan;9(1):187-94. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11804597 11804597] | ||
[[Category: | [[Category: Enterobacteria phage t7]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Atanasiu, C.]] | [[Category: Atanasiu, C.]] | ||
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[[Category: Taylor, P.]] | [[Category: Taylor, P.]] | ||
[[Category: Walkinshaw, M D.]] | [[Category: Walkinshaw, M D.]] | ||
[[Category: all helical]] | [[Category: all helical]] | ||
[[Category: gene regulation]] | [[Category: gene regulation]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:38:38 2008'' | ||
Revision as of 23:38, 30 March 2008
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| , resolution 1.83Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | 0.3 (Enterobacteria phage T7) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of Ocr from Bacteriophage T7
OverviewOverview
We have solved, by X-ray crystallography to a resolution of 1.8 A, the structure of a protein capable of mimicking approximately 20 base pairs of B-form DNA. This ocr protein, encoded by gene 0.3 of bacteriophage T7, mimics the size and shape of a bent DNA molecule and the arrangement of negative charges along the phosphate backbone of B-form DNA. We also demonstrate that ocr is an efficient inhibitor in vivo of all known families of the complex type I DNA restriction enzymes. Using atomic force microscopy, we have also observed that type I enzymes induce a bend in DNA of similar magnitude to the bend in the ocr molecule. This first structure of an antirestriction protein demonstrates the construction of structural mimetics of long segments of B-form DNA.
About this StructureAbout this Structure
1S7Z is a Single protein structure of sequence from Enterobacteria phage t7. Full crystallographic information is available from OCA.
ReferenceReference
Structure of Ocr from bacteriophage T7, a protein that mimics B-form DNA., Walkinshaw MD, Taylor P, Sturrock SS, Atanasiu C, Berge T, Henderson RM, Edwardson JM, Dryden DT, Mol Cell. 2002 Jan;9(1):187-94. PMID:11804597
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