1c03: Difference between revisions
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==CRYSTAL STRUCTURE OF YPD1P (TRICLINIC FORM)== | ==CRYSTAL STRUCTURE OF YPD1P (TRICLINIC FORM)== | ||
<StructureSection load='1c03' size='340' side='right' caption='[[1c03]], [[Resolution|resolution]] 2.30Å' scene=''> | <StructureSection load='1c03' size='340' side='right' caption='[[1c03]], [[Resolution|resolution]] 2.30Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1c03]] is a 4 chain structure | <table><tr><td colspan='2'>[[1c03]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C03 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1C03 FirstGlance]. <br> | ||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2a0b|2a0b]]</td></tr> | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2a0b|2a0b]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1c03 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c03 OCA], [http://pdbe.org/1c03 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1c03 RCSB], [http://www.ebi.ac.uk/pdbsum/1c03 PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1c03 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c03 OCA], [http://pdbe.org/1c03 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1c03 RCSB], [http://www.ebi.ac.uk/pdbsum/1c03 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1c03 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
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<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
</jmolCheckbox> | </jmolCheckbox> | ||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/ | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1c03 ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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</div> | </div> | ||
<div class="pdbe-citations 1c03" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 1c03" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Lee, J Y]] | |||
[[Category: Lee, J | [[Category: Lee, M G]] | ||
[[Category: Lee, M | [[Category: Song, H K]] | ||
[[Category: Song, H | [[Category: Suh, S W]] | ||
[[Category: Suh, S | |||
[[Category: Four helical bundle]] | [[Category: Four helical bundle]] | ||
[[Category: Signal transduction]] | [[Category: Signal transduction]] | ||
[[Category: Signaling protein]] | [[Category: Signaling protein]] | ||
Revision as of 14:12, 1 February 2017
CRYSTAL STRUCTURE OF YPD1P (TRICLINIC FORM)CRYSTAL STRUCTURE OF YPD1P (TRICLINIC FORM)
Structural highlights
Function[YPD1_YEAST] Phosphorelay intermediate protein that is part of the branched SLN1-YPD1-SKN7/SSK1 two-component regulatory system, which controls activity of the HOG1 pathway and gene expression in response to changes in the osmolarity of the extracellular environment. Catalyzes the phosphoryl group transfer from the membrane-bound osmosensing histidine kinase SLN1 to two distinct response regulator proteins, SSK1 in the cytoplasm, and transcription factor SKN7 in the nucleus.[1] [2] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed"Two-component" phosphorelay signal transduction systems constitute a potential target for antibacterial and antifungal agents, since they are found exclusively in prokaryotes and lower eukaryotes (yeast, fungi, slime mold, and plants) but not in mammalian organisms. Saccharomyces cerevisiae Ypd1p, a key intermediate in the osmosensing multistep phosphorelay signal transduction, catalyzes the phosphoryl group transfer between response regulators. Its 1.8 A structure, representing the first example of a eukaryotic phosphorelay protein, contains a four-helix bundle as in the HPt domain of Escherichia coli ArcB sensor kinase. However, Ypd1p has a 44-residue insertion between the last two helices of the helix bundle. The side-chain of His64, the site of phosphorylation, protrudes into the solvent. The structural resemblance between Ypd1p and ArcB HPt domain suggests that both prokaryotes and lower eukaryotes utilize the same basic protein fold for phosphorelay signal transduction. This study sheds light on the best characterized eukaryotic phosphorelay system. Insights into eukaryotic multistep phosphorelay signal transduction revealed by the crystal structure of Ypd1p from Saccharomyces cerevisiae.,Song HK, Lee JY, Lee MG, Moon J, Min K, Yang JK, Suh SW J Mol Biol. 1999 Nov 5;293(4):753-61. PMID:10543964[3] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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