1s3t: Difference between revisions
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|PDB= 1s3t |SIZE=350|CAPTION= <scene name='initialview01'>1s3t</scene>, resolution 2.10Å | |PDB= 1s3t |SIZE=350|CAPTION= <scene name='initialview01'>1s3t</scene>, resolution 2.10Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand= | |LIGAND= <scene name='pdbligand=BO3:BORIC+ACID'>BO3</scene>, <scene name='pdbligand=CXM:N-CARBOXYMETHIONINE'>CXM</scene>, <scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/Urease Urease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.5 3.5.1.5] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Urease Urease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.5 3.5.1.5] </span> | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY=[[1ubp|1UBP]], [[2ubp|2UBP]], [[3ubp|3UBP]], [[4ubp|4UBP]], [[1ie7|1IE7]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s3t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s3t OCA], [http://www.ebi.ac.uk/pdbsum/1s3t PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1s3t RCSB]</span> | |||
}} | }} | ||
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[[Category: Rypniewski, W R.]] | [[Category: Rypniewski, W R.]] | ||
[[Category: Wilson, K S.]] | [[Category: Wilson, K S.]] | ||
[[Category: bacillus pasteurii]] | [[Category: bacillus pasteurii]] | ||
[[Category: borate]] | [[Category: borate]] | ||
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[[Category: urease]] | [[Category: urease]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:37:00 2008'' | ||
Revision as of 23:37, 30 March 2008
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| , resolution 2.10Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , | ||||||
| Activity: | Urease, with EC number 3.5.1.5 | ||||||
| Related: | 1UBP, 2UBP, 3UBP, 4UBP, 1IE7
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
BORATE INHIBITED BACILLUS PASTEURII UREASE CRYSTAL STRUCTURE
OverviewOverview
The structure of the complex of urease, a Ni-containing metalloenzyme, with boric acid was determined at 2.10 A resolution. The complex shows the unprecedented binding mode of the competitive inhibitor to the dinuclear metal center, with the B(OH)3 molecule bridging the Ni ions and leaving in place the bridging hydroxide. Boric acid can be considered a substrate analogue of urea, and the structure supports the proposal that the Ni-bridging hydroxide acts as the nucleophile in the enzymatic process of urea hydrolysis.
About this StructureAbout this Structure
1S3T is a Protein complex structure of sequences from Sporosarcina pasteurii. Full crystallographic information is available from OCA.
ReferenceReference
Molecular details of urease inhibition by boric acid: insights into the catalytic mechanism., Benini S, Rypniewski WR, Wilson KS, Mangani S, Ciurli S, J Am Chem Soc. 2004 Mar 31;126(12):3714-5. PMID:15038715
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