1s3k: Difference between revisions
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|PDB= 1s3k |SIZE=350|CAPTION= <scene name='initialview01'>1s3k</scene>, resolution 1.90Å | |PDB= 1s3k |SIZE=350|CAPTION= <scene name='initialview01'>1s3k</scene>, resolution 1.90Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | |LIGAND= <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s3k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s3k OCA], [http://www.ebi.ac.uk/pdbsum/1s3k PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1s3k RCSB]</span> | |||
}} | }} | ||
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[[Category: Ramsland, P A.]] | [[Category: Ramsland, P A.]] | ||
[[Category: Scott, A M.]] | [[Category: Scott, A M.]] | ||
[[Category: antigen binding fragment]] | [[Category: antigen binding fragment]] | ||
[[Category: beta barrel]] | [[Category: beta barrel]] | ||
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[[Category: immunoglobulin fold]] | [[Category: immunoglobulin fold]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:36:50 2008'' | ||
Revision as of 23:36, 30 March 2008
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| , resolution 1.90Å | |||||||
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| Ligands: | , , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of a Humanized Fab (hu3S193) in Complex with the Lewis Y Tetrasaccharide
OverviewOverview
Antibodies targeting human epithelial carcinomas bearing Lewis Y (Le(y)) carbohydrate antigens provide a striking illustration of convergent immune recognition. We report a 1.9A resolution crystal structure of the Fab of a humanized antibody (hu3S193) in complex with the Le(y) tetrasaccharide, Fuc(alpha 1-->2)Gal(beta 1-->4)[Fuc(alpha 1-->3)]GlcNAc. Comparisons of the hu3S193 and BR96 antibodies bound to Le(y) tumor antigens revealed extremely similar mechanisms for recognition of the carbohydrate epitopes. Solvent plays a critical role in hu3S193 antibody binding to the Le(y) carbohydrate epitope. Specificity for Le(y) is maintained because a conserved pocket accepts an N-acetyl group of the core Gal(beta 1-->4)GlcNAc disaccharide. Closely related blood-group determinants (Le(a) and Le(b)) cannot enter the specificity pocket, making the Le(y) antibodies promising candidates for immunotherapy of epithelial cancer.
About this StructureAbout this Structure
1S3K is a Protein complex structure of sequences from Mus musculus and homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Structural convergence of antibody binding of carbohydrate determinants in Lewis Y tumor antigens., Ramsland PA, Farrugia W, Bradford TM, Mark Hogarth P, Scott AM, J Mol Biol. 2004 Jul 16;340(4):809-18. PMID:15223322
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