1s2g: Difference between revisions
No edit summary |
No edit summary |
||
| Line 5: | Line 5: | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=3D1:5-(6-AMINO-PURIN-9-YL)-2-HYDROXYMETHYL-TETRAHYDRO-FURAN-3-OL'>3D1</scene> | |LIGAND= <scene name='pdbligand=3D1:5-(6-AMINO-PURIN-9-YL)-2-HYDROXYMETHYL-TETRAHYDRO-FURAN-3-OL'>3D1</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/Nucleoside_deoxyribosyltransferase Nucleoside deoxyribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.6 2.4.2.6] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Nucleoside_deoxyribosyltransferase Nucleoside deoxyribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.6 2.4.2.6] </span> | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY=[[1f8y|1F8Y]], [[1s2d|1S2D]], [[1s2i|1S2I]], [[1s2l|1S2L]], [[1s3f|1S3F]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s2g OCA], [http://www.ebi.ac.uk/pdbsum/1s2g PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1s2g RCSB]</span> | |||
}} | }} | ||
| Line 26: | Line 29: | ||
[[Category: Ealick, S E.]] | [[Category: Ealick, S E.]] | ||
[[Category: Kaminski, P A.]] | [[Category: Kaminski, P A.]] | ||
[[Category: 2'-deoxyadenosine]] | [[Category: 2'-deoxyadenosine]] | ||
[[Category: 2'-purine deoxyribosyltansferase]] | [[Category: 2'-purine deoxyribosyltansferase]] | ||
[[Category: ptd]] | [[Category: ptd]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:36:24 2008'' | ||
Revision as of 23:36, 30 March 2008
| |||||||
| , resolution 2.10Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Activity: | Nucleoside deoxyribosyltransferase, with EC number 2.4.2.6 | ||||||
| Related: | 1F8Y, 1S2D, 1S2I, 1S2L, 1S3F
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Purine 2'deoxyribosyltransferase + 2'-deoxyadenosine
OverviewOverview
The structure of class I N-deoxyribosyltransferase from Lactobacillus helveticus was determined by X-ray crystallography. Unlike class II N-deoxyribosyltransferases, which accept either purine or pyrimidine deoxynucleosides, class I enzymes are specific for purines as both the donor and acceptor base. Both class I and class II enzymes are highly specific for deoxynucleosides. The class I structure reveals similarities with the previously determined class II enzyme from Lactobacillus leichmanni [Armstrong, S. A., Cook, W. J., Short, S. A., and Ealick, S. E. (1996) Structure 4, 97-107]. The specificity of the class I enzyme for purine deoxynucleosides can be traced to a loop (residues 48-62), which shields the active site in the class II enzyme. In the class I enzyme, the purine base itself shields the active site from the solvent, while the smaller pyrimidine base cannot. The structure of the enzyme with a bound ribonucleoside shows that the nucleophilic oxygen atom of Glu101 hydrogen bonds to the O2' atom, rendering it unreactive and thus explaining the specificity for 2'-deoxynucleosides. The structure of a ribosylated enzyme intermediate reveals movements that occur during cleavage of the N-glycosidic bond. The structures of complexes with substrates and substrate analogues show that the purine base can bind in several different orientations, thus explaining the ability of the enzyme to catalyze alternate deoxyribosylation at the N3 or N7 position.
About this StructureAbout this Structure
1S2G is a Single protein structure of sequence from Lactobacillus helveticus. Full crystallographic information is available from OCA.
ReferenceReference
Structures of purine 2'-deoxyribosyltransferase, substrate complexes, and the ribosylated enzyme intermediate at 2.0 A resolution., Anand R, Kaminski PA, Ealick SE, Biochemistry. 2004 Mar 9;43(9):2384-93. PMID:14992575
Page seeded by OCA on Sun Mar 30 23:36:24 2008