Endonuclease: Difference between revisions
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'''Endonuclease''' (ENN) cleaves phosphodiester bond within polynucleotide chain. ENN cleaves DNA at a restriction site which is usually a 6-nucleotide palindrome. ENN is restriction site–specific. Various types of ENN differ by their mechanism of action. ENN is used in genetic engineering to make recombinant DNA. ENN requires a restriction site and a cleavage pattern. '''ENN-I''' operates on DNA with separate restriction site and cleavage pattern, while '''ENN-II''' operates on overlapping restriction site and cleavage pattern. Some ENNs are encoded within introns thus facilitating their mobility. These ENNs or inteins are designated I-ENN<ref>PMID:12483517</ref>.<br /> | '''Endonuclease''' (ENN) cleaves phosphodiester bond within polynucleotide chain. ENN cleaves DNA at a restriction site which is usually a 6-nucleotide palindrome. ENN is restriction site–specific. Various types of ENN differ by their mechanism of action. ENN is used in genetic engineering to make recombinant DNA. ENN requires a restriction site and a cleavage pattern. '''ENN-I''' operates on DNA with separate restriction site and cleavage pattern, while '''ENN-II''' operates on overlapping restriction site and cleavage pattern. Some ENNs are encoded within introns thus facilitating their mobility. These ENNs or inteins are designated I-ENN<ref>PMID:12483517</ref>.<br /> | ||
The '''Cas ENN''' proteins are part of '''CRISPR/Cas''' prokaryotic immune system which confers protection from foreign genetic elements like viruses. The '''CRISPR''' ('''C'''lustered '''R'''egularly '''I'''nterspersed '''S'''hort '''P'''alindromic '''R'''epeats) are DNA loci which are found in ca. 40% of the bacteria. The CRISPR/Cas system is being used lately as gene editing tool<ref>PMID:20056882</ref>. For more details see<br /> | The '''Cas ENN''' proteins are part of '''CRISPR/Cas''' prokaryotic immune system which confers protection from foreign genetic elements like viruses. The '''CRISPR''' ('''C'''lustered '''R'''egularly '''I'''nterspersed '''S'''hort '''P'''alindromic '''R'''epeats) are DNA loci which are found in ca. 40% of the bacteria. The CRISPR/Cas system is being used lately as gene editing tool<ref>PMID:20056882</ref>. For more details see<br /> | ||
* [[CRISPR-Cas Part I]]<br /> | |||
* [[CRISPR-Cas Part II]]<br /> | |||
* [[Cas9]]<br /> | * [[Cas9]]<br /> | ||
* [[CRISPR-Cas9]]<br /> | * [[CRISPR-Cas9]]<br /> | ||