User:Manon Raiffort/Sandbox: Difference between revisions

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Von Willebrand Factor is a blood multimeric glycoprotein which is synthetized by endothelials cells of the blood vessels and megakaryocytes of the bone marrow. It is found as big multimer in platelet and endothelials cells. In the plasma it is cleaved by metalloprotease into smaller multimers. Each multimer is composed of numerous dimers made of two identical subunits which contains several domains : D’-D3-A1-A2-A3-D4-B1-B2-B3-C1-C2-C3CK. It is the biggest protein in the blood.

Von Willebrand Factor is a blood multimeric glycoprotein which is synthetized by endothelials cells of the blood vessels and megakaryocytes of the bone marrow. It is found as big multimer in platelet and endothelials cells. It is the biggest protein in the blood.

When a blood vessel is damaged , some mechanisms come into play in order to stop the bleeding. It is called hemostasis process and the Von Willebrand factor is involved into it.

== Synthesis ==

The protein is encoded by a gene of 52 exons localized on the chromosome 12.

vWF precursor is synthetized as a very large protein in endothelium cells and megekaryocytes.

It undergoes post-translationnal events :

* delete the signal peptide and a large propeptide

* formation of intra and inter chains disulfide bonds

* glycosylations

Dimers are formed in the endoplasmic reticulum (ER) thanks to disulfide bonds formation of C-terminal. These dimers create some multimers via formation N-terminal disulfide bridges between them in the Golgi apparatus.

The mature vWF is synthetized in endothelium cells. It can be immediately secreted (constitutive way) or stocked in the Weibel-Palade body (regulated way). In plasma, vWF exists as a mixture of disulfide bonded multimers with a size between 500 kDa and 10 000 kDa. So, it exists a size distribution for a normal haemostasis.

In the plasma it is cleaved by metalloprotease into smaller multimers. Each multimer is composed of numerous dimers made of two identical subunits which contains several domains : D’-D3-A1-A2-A3-D4-B1-B2-B3-C1-C2-C3CK.

== Function ==

Von Willebrand factor has two mainly functions in diferent phenomenon :

- Coagulation : act as a carrier for the plasma procoagulant co-enzyme, factor VIII. Factor VIII bound to VWF is protected from

* Coagulation : act as a carrier for the plasma procoagulant co-enzyme, factor VIII. Factor VIII bound to VWF is protected from

inactivation by proteases. Factor VIII is released from VWF by the action of thrombin.

inactivation by proteases. Factor VIII is released from VWF by the action of thrombin. The factor VIII is bound to two domains : D'/D3.

- Platelet plug formation : promote platelet adhesion to thrombogenic surfaces and their aggregation

* Platelet plug formation : promote platelet adhesion to thrombogenic surfaces and their aggregation. Receptor Gp-1b-IX-V

This factor is also bound to :

~~- collagen~~ when the blood vessel is damaged by its A1 and A3 domains

* [[Collagen]] when the blood vessel is damaged by its A1 and A3 domains. A3 can fixe to collagen I or III

- Platelet integrins αIIb/β3 by the C1 domain (which contains the RGD pattern)

* Platelet integrins αIIb/β3 by the C1 domain (which contains the RGD pattern)

== Disease ==

Idea : touffe (clump) de plaquette

== Relevance ==

== Structural highlights ==

~~[[Image:structure I VWF.jpg]]~~

This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.

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 <StructureSection load='1ATZ' size='340' side='right' caption='Caption for this structure' scene=''>
-Von Willebrand Factor is a blood multimeric glycoprotein which is synthetized by endothelials cells of the blood vessels and megakaryocytes of the bone marrow. It is found as big multimer in platelet and endothelials cells. In the plasma it is cleaved by metalloprotease into smaller multimers. Each multimer is composed of numerous dimers made of two identical subunits which contains several domains : D’-D3-A1-A2-A3-D4-B1-B2-B3-C1-C2-C3CK.  It is the biggest protein in the blood.
+Von Willebrand Factor is a blood multimeric glycoprotein which is synthetized by endothelials cells of the blood vessels and megakaryocytes of the bone marrow. It is found as big multimer in platelet and endothelials cells.   It is the biggest protein in the blood.
 When a blood vessel is damaged , some mechanisms come into play in order to stop the bleeding. It is called hemostasis process and the Von Willebrand factor is involved into it.
 == Synthesis ==
+The protein is encoded by a gene of 52 exons localized on the chromosome 12.
+vWF precursor is synthetized as a very large protein in endothelium cells and megekaryocytes.
+It undergoes post-translationnal events :
+* delete the signal peptide and a large propeptide
+* formation of intra and inter chains disulfide bonds
+* glycosylations
+Dimers are formed in the endoplasmic reticulum (ER) thanks to disulfide bonds formation of C-terminal. These dimers create some multimers via formation N-terminal disulfide bridges between them in the Golgi apparatus.
+The mature vWF is synthetized in endothelium cells. It can be immediately secreted (constitutive way) or stocked in the Weibel-Palade body (regulated way). In plasma, vWF exists as a mixture of disulfide bonded multimers with a size between 500 kDa and 10 000 kDa. So, it exists a size distribution for a normal haemostasis.
+In the plasma it is cleaved by metalloprotease into smaller multimers. Each multimer is composed of numerous dimers made of two identical subunits which contains several domains : D’-D3-A1-A2-A3-D4-B1-B2-B3-C1-C2-C3CK.
 == Function ==
 Von Willebrand factor has two mainly functions in diferent phenomenon :
-- Coagulation : act as a carrier for the plasma procoagulant co-enzyme, factor VIII. Factor VIII bound to VWF is protected from
+* Coagulation : act as a carrier for the plasma procoagulant co-enzyme, factor VIII. Factor VIII bound to VWF is protected from
-inactivation by proteases. Factor VIII is released from VWF by the action of thrombin.
+inactivation by proteases. Factor VIII is released from VWF by the action of thrombin. The factor VIII is bound to two domains : D'/D3.
-- Platelet plug formation : promote platelet adhesion to thrombogenic surfaces and their aggregation
+* Platelet plug formation : promote platelet adhesion to thrombogenic surfaces and their aggregation. Receptor Gp-1b-IX-V
 This factor is also bound to :
-- collagen when the blood vessel is damaged by its A1 and A3 domains
+* [[Collagen]] when the blood vessel is damaged by its A1 and A3 domains. A3 can fixe to collagen I or III
-- Platelet integrins αIIb/β3  by the C1 domain (which contains the RGD pattern)
+* Platelet integrins αIIb/β3  by the C1 domain (which contains the RGD pattern)
 == Disease ==
+Idea : touffe (clump) de plaquette
 == Relevance ==
 == Structural highlights ==
-[[Image:structure I VWF.jpg]]
 This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.