1rfs: Difference between revisions
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|PDB= 1rfs |SIZE=350|CAPTION= <scene name='initialview01'>1rfs</scene>, resolution 1.83Å | |PDB= 1rfs |SIZE=350|CAPTION= <scene name='initialview01'>1rfs</scene>, resolution 1.83Å | ||
|SITE= <scene name='pdbsite=FSB:Fe-S+Binding+Site'>FSB</scene> | |SITE= <scene name='pdbsite=FSB:Fe-S+Binding+Site'>FSB</scene> | ||
|LIGAND= <scene name='pdbligand=FES:FE2/S2 (INORGANIC) CLUSTER'>FES</scene> | |LIGAND= <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rfs OCA], [http://www.ebi.ac.uk/pdbsum/1rfs PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1rfs RCSB]</span> | |||
}} | }} | ||
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[[Category: Smith, J L.]] | [[Category: Smith, J L.]] | ||
[[Category: Zhang, H.]] | [[Category: Zhang, H.]] | ||
[[Category: electron transport]] | [[Category: electron transport]] | ||
[[Category: iron-sulfur protein]] | [[Category: iron-sulfur protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:27:33 2008'' | ||
Revision as of 23:27, 30 March 2008
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| , resolution 1.83Å | |||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
RIESKE SOLUBLE FRAGMENT FROM SPINACH
OverviewOverview
BACKGROUND: The cytochrome b6f complex functions in oxygenic photosynthesis as an integral membrane protein complex that mediates coupled electron transfer and proton translocation. The Rieske [2Fe-2S] protein subunit of the complex functions at the electropositive (p) membrane interface as the electron acceptor for plastoquinol and donor for the cytochrome f subunit, and may have a dynamic role in catalyzing electron and proton transfer at the membrane interface. There are significant structure/function similarities to the cytochrome bc1 complex of the respiratory chain. RESULTS: The 1.83 A crystal structure of a 139-residue C-terminal fragment of the Rieske [2Fe-2S] protein, derived from the cytochrome b6f complex of spinach chloroplasts, has been solved by multiwavelength anomalous diffraction. The structure of the fragment comprises two domains: a small 'cluster-binding' subdomain and a large subdomain. The [2Fe-2S] cluster-binding subdomains of the chloroplast and mitochondrial Rieske proteins are virtually identical, whereas the large subdomains are strikingly different despite a common folding topology. A structure-based sequence alignment of the b6f and bc1 groups of Rieske soluble domains is presented. CONCLUSIONS: The segregation of structural conservation and divergence in the cluster-binding and large subdomains of the Rieske protein correlates with the overall relatedness of the cytochrome b6f and bc1 complexes, in which redox domains in the aqueous p phase are dissimilar and those within the membrane are similar. Distinct sequences and surface charge distributions among Rieske large subdomains may provide a signature for interaction with the p-side oxidant protein and for the pH of the intraorganelle compartment.
About this StructureAbout this Structure
1RFS is a Single protein structure of sequence from Spinacia oleracea. Full crystallographic information is available from OCA.
ReferenceReference
Biological identity and diversity in photosynthesis and respiration: structure of the lumen-side domain of the chloroplast Rieske protein., Carrell CJ, Zhang H, Cramer WA, Smith JL, Structure. 1997 Dec 15;5(12):1613-25. PMID:9438861
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